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Label IT® Nucleic Acid Labeling Kit, Amine – sufficient for modifying 25 µg of nucleic acid

SKU MIR 3925 Categories ,

$324.00

Description

  • Modify Any DNA or RNA Template – Direct, covalent attachment of amine functional groups to any nucleic acid
  • One-step Chemical Method – Easily and consistently control the density of nucleic acid modification
  • Covalent Mechanism – Permanent, non-destructive modification of nucleic acids which can then be conjugated to proteins or peptides, or attached to glass surfaces, or beads or plates

 

Kit Components:
Each Kit contains Label IT® Reagent, Label IT® Reconstitution Solution, Labeling Buffer, Solutions and G50 Spin Columns.

LabelIT General LabelIt Schematic Data Illustration

Label IT® Nucleic Acid Modifying Kit. The Label IT® nucleic acid modifying reagent is composed of three regions: the reactive NH2 modifying group (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT® modifying reagent to any reactive heteroatom within nucleic acids. Attachment of the Label IT® modifying reagent to nucleic acids does not alter the structure of the nucleic acids or affect downstream hybridization performance. Once labeled, the nucleic acids can be coupled to reactive substrates of other chemical entities through the added NH2 reactive groups.

 

LabelIT Nucleic Acid Modifying Kit Amine Labeling Ratio Data

Demonstration of the Functionality of Amine Groups on Modified DNA. Plasmid DNA (5.6 kb) was reacted with Label IT® Amine Reagent at the ratios (w:w) of labeling reagent to DNA indicated and purified by ethanol precipitation. Five µg of the modified DNA, in triplicate, was reacted with 10 mM NHS ester TM-Rhodamine and 100 mM NaHCO3 (pH 8 – 8.5) for 1 hour at room temperature in the dark. The DNA was again ethanol precipitated, washed extensively, and assayed for labeling efficiency.

SKU: MIR 3925

Kit Components

Materials Supplied

The following components are included in the Label IT® Nucleic Acid Modifying Kits

 Kit Component Full Size Trial Size Reagent Cap Color
Label IT® Modifying Reagent 100 μg dried pellet 25 μg dried pellet Red
Reconstitution Solution 100 μl 100 μl Brown
10X Labeling Buffer A 500 μl 100 μl Orange
Denaturation Reagent D1 500 μl 150 μl Blue
Neutralization Buffer N1 500 μl 200 μl White
G50 microspin purification columns 20 5 N/A

Materials required, but not supplied

  • Molecular biology-grade water
  • Nucleic acid sample (starting material)
  • Optional: Materials for EtOH purification
  • Optional: NH2 secondary conjugates or detection reagents

Resources

Specifications

Storage Conditions
All Configurations: Store the Label IT® Reagent at -20°C in both dried pellet and reconstituted form. Store all other supplied reagents at -20°C. Store G50 microspin purification columns at 4°C. DO NOT FREEZE.

Product Guarantee
All Configurations: 1 year (6 months after reconstitution)

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

 

Technical Product Literature

Full Protocols
Label IT Amine Full Labeling Protocol (PDF)

Quick Reference Protocols
Label IT Amine Quick Ref Protocol (PDF)

SDS
Label IT Amine SDS (PDF)

Using transfection reagents and enhancers from Mirus has given our platform a competitive advantage. Increasing efficiency and lowering costs for all of our programs.

Sally Mader, PhD
ZYZ Theraputics