Label IT^® Nucleic Acid Labeling Kit, Amine – sufficient for modifying 100 µg of nucleic acid

Label IT® Nucleic Acid Modifying Kit. The Label IT® nucleic acid modifying reagent is composed of three regions: the reactive NH2 modifying group (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT® modifying reagent to any reactive heteroatom within nucleic acids. Attachment of the Label IT® modifying reagent to nucleic acids does not alter the structure of the nucleic acids or affect downstream hybridization performance. Once labeled, the nucleic acids can be coupled to reactive substrates of other chemical entities through the added NH2 reactive groups.

Demonstration of the Functionality of Amine Groups on Modified DNA. Plasmid DNA (5.6 kb) was reacted with Label IT® Amine Reagent at the ratios (w:w) of labeling reagent to DNA indicated and purified by ethanol precipitation. Five µg of the modified DNA, in triplicate, was reacted with 10 mM NHS ester TM-Rhodamine and 100 mM NaHCO3 (pH 8 – 8.5) for 1 hour at room temperature in the dark. The DNA was again ethanol precipitated, washed extensively, and assayed for labeling efficiency.