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Label IT Nucleic Acid Labeling Kit, MFP488 – labels 100 µg nucleic acid

Efficient, direct, non-enzymatic labeling of DNA and RNA

SKU MIR 7100 Categories ,

$683.00

Description

MFP488 Nucleic Acid Labeling Reagent

  • High Photostability–Facilitates longer time for observation and image capture
  • Widespread Instrument Compatibility– Spectrally similar to fluorescein
  • Stable at Low pH– Fluorescence is maintained over a broad range (pH 4-9)

 

NOTE: All the Label IT® Reagents use the same technology, but each attach different fluorophores with distinct excitation and emission spectra.

 

SKU: MIR 7100

Supporting Data

Label IT® Nucleic Acid Labeling Kits. The Label IT® chemical labeling reagents are composed of three regions: the label (fluorophore or hapten) (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT® reagent to any reactive heteroatom within the nucleic acids. Attachment of the Label IT® Reagents to nucleic acids does not alter the structure of the nucleic acid or affect downstream hybridization performance, and as such, nucleic acids labeled using the Label IT® Reagents can be employed in multiple applications as defined by the researcher.

 

Label IT® MFP488 Labeled Plasmid DNA is Less Sensitive to Photobleaching as Compared to Label IT® Fluorescein Labeled Plasmid DNA. (A) Plasmid DNA was  labeled with Label IT® MFP488 or Label IT® Fluorescein.  Purified DNA was transfected into HeLa cells using the TransIT®-LT1 Transfection Reagent at a 3:1 reagent-to-DNA ratio (vol:wt). Two hours post transfection labeled DNA was visualized using a 63X oil immersion objective on a Zeiss Axiovert Inverted Fluorescence microscope. Images were captured before and after a 2 minute photobleaching period from a FITC light source. (B) The ratio (X-axis) of the brightness of individual puncta was calculated by dividing the photobleached value for fluorescence brightness by the value obtained from non-photobleached values for four separate images using Image J software (NIH), the average and standard deviation are shown. Photobleaching was also measured after 5 minutes for Label IT-MFP488 labeled plasmid DNA and the signal was still 4-fold higher than Label IT® Fluorescein labeled plasmid DNA at 2 minutes (data not shown).

 

Label IT® MFP488 Labeled Plasmid DNA is More Resistant to the Effects of Low pH as Compared to Label IT® Fluorescein Labeled Plasmid DNA. Plasmid DNA was labeled at a low density, 1 label per 65 bp, using Label IT® MFP488 or Label IT® Fluorescein Nucleic Acid Labeling Kits. Purified DNA was subsequently diluted in various pH buffers (50 mM HEPES, pH 8, 100 mM MES, pH 5.4, and 100 mM MES, pH 3). Fluorescence was measured using a Tecan Genios Fluorometer and normalized to the respective control measured at 50 mM HEPES, pH 8.

 

Resources

Specifications

Storage Conditions
All Configurations: Store the Label IT® Reagent at -20°C in both dried pellet and reconstituted form. Store all other supplied reagents at -20°C. Store G50 microspin purification columns at 4°C. DO NOT FREEZE.

Product Guarantee
All Configurations: 1 year (6 months after reconstitution)

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

 

Technical Product Literature

Full Protocols
Label IT Kit Full Labeling Protocol (PDF)

Quick Reference Protocols
Label IT Kit Quick Ref Protocol (PDF)

SDS
Label IT Kit SDS (PDF)

FAQs

See the Label IT FAQs

Using transfection reagents and enhancers from Mirus has given our platform a competitive advantage. Increasing efficiency and lowering costs for all of our porgrams.

 

Sally Mader, PhD
ZYZ Theraputics