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Label IT Nucleic Acid Labeling Kit, Fluorescein – labels 25 µg nucleic acid

Efficient, direct, non-enzymatic labeling of DNA and RNA

SKU MIR 3225 Categories ,

$275.00

Description

Nucleic Acid Labeling Reagents

  • Label Any DNA or RNA Template–Suitable for a wide range of applications
  • One-step Chemical Method– Easily and consistently control the labeling reactions
  • Adjustable Labeling Density– Achieve high sensitivity with optimally labeled DNA and RNA
  • Covalent Mechanism– Permanent, non-destructive modification of nucleic acid residues is ideal for many diverse applications; labels do not impact hybridization performance

 

NOTE: All the Label IT® Reagents use the same technology, but each attach different fluorophores with distinct excitation and emission spectra.

 

 

SKU: MIR 3225

Supporting Data

 

Label IT® Nucleic Acid Labeling Kits. The Label IT® chemical labeling reagents are composed of three regions: the label (fluorophore or hapten) (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT® reagent to any reactive heteroatom within the nucleic acids. Attachment of the Label IT® Reagents to nucleic acids does not alter the structure of the nucleic acid or affect downstream hybridization performance, and as such, nucleic acids labeled using the Label IT® Reagents can be employed in multiple applications as defined by the researcher.

 

Non-destructive Direct Labeling of Nucleic Acid. Mouse total RNA was labeled with Label IT® Cy®3 (B) electrophoresed and analyzed alongside unlabeled total RNA (A) on an Agilent RNA 6000 Nano chip. RNA Integrity Number (RIN) is approximately 9.0 for both labeled and unlabeled samples.

 

Labeling Density can be Easily Controlled with the Label IT® Reagents. Plasmid DNA was labeled with increasing ratios (w:w) of Label IT® CX-Rhodamine to DNA. The extent of labeling was estimated, and normalized to the amount of recovered DNA (# labels/100 base pairs), using two different criteria: fluorescence intensity of the attached rhodamine (excitation at 585 nm, emission at 610 nm), and visible absorbance at 585 nm. The significance of quenching, at the higher labeling ratios, becomes apparent in the fluorimetric estimations of labeling density.

Resources

Specifications

Storage Conditions
All Configurations: Store the Label IT® Reagent at -20°C in both dried pellet and reconstituted form. Store all other supplied reagents at -20°C. Store G50 microspin purification columns at 4°C. DO NOT FREEZE.

Product Guarantee
All Configurations: 1 year (6 months after reconstitution)

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

 

Technical Product Literature

Full Protocol
Label IT Kit Full Labeling Protocol (PDF)

Quick Reference Protocol
Label IT Kit Quick Ref Protocol (PDF)

SDS
Label IT Kit SDS (PDF)

FAQs

See the Label IT FAQs

Using transfection reagents and enhancers from Mirus has given our platform a competitive advantage. Increasing efficiency and lowering costs for all of our porgrams.

 

Sally Mader, PhD
ZYZ Theraputics