Description
flashBAC™ Baculovirus Expression Systems are a streamlined platform for the production of recombinant baculoviruses. Genetic optimization of the Autographa californica nucleopolyhedrovirus (AcMNPV) genome yields recombinant virus in a single step and eliminates the need for separation of recombinant and parental virus by plaque purification. flashBAC™ systems and pOET Insect and BacMam Transfer Plasmids are optimized for use with TransIT®-Insect Transfection Reagent.
Benefits of the flashBAC™ system include:
- Ease-of-Use – Single-step baculovirus generation in insect cells, no plaque purification required
- Compatibility – Ready to use with most transfer vectors (PDF), including BacMam
- More Protein – High recombinant protein yields
Supporting Data
flashBAC™ Systems offered by Mirus Bio:
System |
Description |
flashBAC™ |
The original flashBAC™ expression vector. Protein expression and secretion is improved by deletion of the chitinase gene from the AcMNPV genome. Optimal for expression of most nuclear or cytoplasmic proteins. |
flashBAC™ ULTRA |
The most advanced flashBAC™ vector system, optimized by deletions in the chitinase, cathepsin, p10, p74 and p26 genes. The ideal system for the most difficult to express proteins including nuclear, cytoplasmic, membrane and secreted proteins. |
Additional details on the genetic modifications included in the flashBAC™ systems are described in Hitchman et al. 2010. |
How does the flashBAC™ Baculorvirus Expression System work?
flashBAC™ Systems and pOET vectors are sold by Mirus Bio through partnership with Oxford Expression Technologies, Oxford, UK.
Resources
Specifications
Storage Conditions
All Configurations: Store flashBAC™ DNA at 4°C and control plasmid at 4°C or –20°C
Product Guarantee
All Configurations: 1 year
Usage Statement
All Configurations: For Research Use Only.
Animal Origin Statement
All Configurations: This product is animal origin free.
Technical Product Literature
Full Protocol
flashBAC™ System Transfection Protocol (PDF)
Quick Reference Protocol
flashBAC™ System Quick Ref Protocol (PDF)
SDS
flashBAC™ System SDS (PDF)