Search Results for: TransIt-Virusgen transfection reagent – Page 3

Hijacking Viruses: Optimizing Lentivirus-based Cell Engineering | A podcast

Researchers commonly employ lentiviruses to modify cells genetically. However, they must overcome several challenges when using these viruses in the laboratory or clinic.

Fill out the form to listen to the latest LabTalk Podcast produced by The Scientist, where Filippo Rossignoli, instructor in neurosurgery at the Center for Stem Cell and Translational Immunotherapy at Brigham and Women’s Hospital and Harvard Medical School, discusses using lentiviruses to engineer cell therapies and the troubleshooting process he undertook to optimize viral production and infection.

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Transfecting Insect Cells, Pt. 4

Howdy everyone! What we have all BEEn waiting for: the final installment of our ‘Transfecting Insect Cells’ series with Dr. Bees.

So far, we have discussed common insect cell lines and TransIT®-Insect (Part 1), producing baculovirus with flashBAC™ (Part 2) and key features of pOET Transfer Plasmids (Part 3). Today in Part 4, we will bug out on how baculovirus can be used to produce virus-like particles (VLPs) and other kinds of viral vectors, such as AAV!

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Chromothripsis and the Epstein-Barr Virus

Chromothripsis is a term used to describe chromosome shattering. In this SNiP, we highlight a recent study by Li et al. that revealed a link between Epstein-Barr Virus (EBV) infection and chromosomal instability. The authors identify a locus on chromosome 11q23 that is susceptible to binding by multiple copies of the EBV protein EBNA1. They propose a mechanism by which this concentrated binding and subsequent chromosomal breakage could explain the association between EBV and a variety of cancers.

And, did we mention that >90% of adults worldwide live with EBV? Read on to learn more.

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Targeting STMN2 Splicing to Treat ALS

Today’s TransMission is a SNiP (Small News in Pieces) about stathmin-2, which is encoded by the STMN2 gene. No, this is not a pun on a Jason Statham action movie. STMN2 is a potential drug target for amyotrophic lateral sclerosis (ALS). In this SNiP, we highlight how Baughn & Melamed et al. modulate splicing of STMN2 mRNA with antisense oligonucleotide- and CRISPR-based approaches. The strategies and tools introduced in their study will be useful in the development of gene therapies to treat ALS and related neurodegenerative diseases.

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Understand Viral Titer & Quality

The cell and gene therapy field has burgeoned in the last decade with drug candidate treatments for many different diseases. Determining the correct patient dosage has become more important than ever to extend the reach of the multi-million-dollar manufacturing runs required to generate these viral therapies.

Genome copies (GC), capsids, GC/capsid ratios, percent full, functional titering… what does it all mean? In this Transfection 101, we summarize commonly used approaches to verify that manufactured viral vectors are of high quality and functionality, appropriate for use in patients.

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Storage of TransITs

Proper storage and handling of TransIT® reagents are required to maintain optimal transfection performance. This is a quick reference page for storage and handling of Mirus TransIT® Transfection Reagents and Kits.

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Leveraging Avian AAV in Pekin Duck Research

Though typically seen in reference to human disease, AAV has applications apart from therapeutics for otherwise untreatable maladies. Today we SNiP a proof-of-concept study that features a unique use for TransIT-VirusGEN® in production of avian AAV for the transduction of Pekin duck cells in vitro and in vivo.

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