Applications | Stem Cell Transfection
Applications | Stem Cell Transfection Stem Cell Solutions DNA Transfection RNA Transfection Electroporation Products Stem Cell Transfection Solutions Mirus Bio reagents enable high efficiency transfection
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Applications | Electroporation
Applications | Electroporation with Ingenio® Ingenio Benefits Competitive Comparison RNA Electroporation Products The Best Choice for High Efficiency Electroporation Mirus Bio offers a full range
Applications | Co-transfection
Co-transfection or co-delivery of multiple nucleic acids – either similar or different in nature is a technique routinely employed by cell biologists to address increasingly complex experimental design and applications.
Applications | CRISPR/Cas9 Genome Editing
Applications | Genome Editing Using CRISPR/Cas9 CRISPR/Cas pDNA + gRNA Transfection mRNA + gRNA Transfection RNP Delivery Products Transfection Methods for CRISPR/Cas9 Genome Editing TRANSFECTION
Applications | High Throughput Transfection
Applications | High Throughput Transfection High Throughput Reverse Transfection of DNA RNAi Screening Protocol HT Citations and Reagents Products High Throughput Transfection Advances in high
Applications | RNAi Gene Silencing
Applications | RNAi Gene Silencing TransIT for Gene Silencing siRNA Mediated Pathway miRNA Mediated Pathway shRNA Mediated Pathway Products TransIT® Transfection Reagents for Gene Silencing
Applications | Antibody/Protein Production
Applications | Antibody/Protein Production Overview Suspension CHO Cells Suspension HEK-293 Cells Transfect Expi293F Cells Products Biotherapeutic Protein Production in CHO and 293 Cells Achieve high
Enrich CRISPR’d Cells with Label IT®
Today’s TransMission is a SNiP of one of our favorite papers featuring Label IT®. Nasri and Mir et al. describe an elegant use for Label IT® in enriching CRISPR/Cas-edited cells. For use in the clinic, it is highly desirable to limit the number of nonedited cells that may compete with the therapeutic, modified cells. Read on to learn how Label IT® was used to enhance the CRISPR genome-editing workflow!
Regulate pLIVE® Expression Level
pLIVE® is a vector system designed for long-term expression in the livers of laboratory rodents. In this Tips from the Bench, we share how gene expression levels can be modulated by changing the relative position of the gene of interest within the pLIVE® multiple cloning site. We also discuss testing the pLIVE® construct in vitro before beginning in vivo studies.
Transfection Efficiency
“How successful was your transfection experiment?” Transfection efficiency is a measure used to answer this question. Because success can be defined in multiple ways, how to calculate transfection efficiency will depend on the type of transfection experiment.
In this TransMission Transfection 101 we will cover: the many meanings of transfection efficiency, common reporter assays to measure transfection efficiency, and how to improve transfection efficiency.