Howdy everyone! Dr. Bees here. So far, we have discussed common insect cell lines and the insect-specific transfection reagent TransIT®-Insect (Part 1) and producing baculovirus with flashBAC™ (Part 2). Today in Part 3, we will do a quick flyover of the pOET Transfer Plasmids, which complement flashBAC™ DNA like bees and honey!
Howdy everyone! Dr. Bees is back with the 2nd installment of our insect transfection series, and bee-lieve me you will enjoy this one. Last time, we covered commonly transfected insect cell lines. Today we are going to focus on flashBAC™–what it is and how to use it for producing baculovirus.
Howdy everyone! Guest writing for the TransMission is one of our field application specialists, Dr. Bees… which is a nom-de-plume, of course!
Starting the month of February for you love bugs and valentines, Dr. Bees will be doing a series of insect-themed posts, including transfection solutions that Mirus has developed for insect cells. In Part 1, we begin by introducing the cast of characters and the critical cell biology of insects.
High Yield Transfection and Baculovirus Production in Insect Cells [Madison, WI December 11, 2013] If low protein expression in insect cells is bugging you, consider