Search Results for: CHOgro High Yield Expression System – Page 2
TransIT-PRO® Transfection Reagent – 1 mL (without boost)
High, Reproducible Protein Yield in Suspension CHO and 293 Cells
TransIT-PRO® Transfection Reagent – 10 mL (without boost)
High, Reproducible Protein Yield in Suspension CHO and 293 Cells
Applications | Antibody/Protein Production
Applications | Antibody/Protein Production Overview Suspension CHO Cells Suspension HEK-293 Cells Transfect Expi293F Cells Products Biotherapeutic Protein Production in CHO and 293 Cells Achieve high
Generate Stable CHO Cell Lines
Using the CHOgro® High Yield Expression System, exceptional yields of recombinant protein can be obtained by transient transfection of suspension CHO cells. Though transgene expression ceases for the vast majority of cells within one to two weeks post-transfection, a small fraction of transfected CHO cells continues to stably express the recombinant protein through random chromosomal integration of the transfected gene. In this Tip from the Bench, we share a protocol for generating stable cell lines through selection and expansion of CHO cells which produce high levels of recombinant protein.
Maintain Suspension CHO Cells
Suspension CHO cells have emerged as one of the most important cell lines for protein expression in biomanufacturing for their capacity to grow to high cell density in serum-free medium. Successful cell growth of these popular cells is dependent on several factors, which include media composition, culture format and appropriate cell maintenance. In today’s Tip from the Bench, we share a few tips for keeping your suspension CHO cells in transfection-ready shape!
Protein Production
Recombinant protein and mAb production Get the titer you need from your cell line of choice We’ve designed an advanced transfection solution for industry-leading protein
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About Us At Mirus Bio, Innovation Never Ends The number of applications that rely on delivery of nucleic acids into cells continues to expand and
Crossing the BBB with Nanobodies
The blood brain barrier (BBB) protects our brains from circulating pathogens and inflammatory cells. However, it also prevents the delivery of therapeutics into the brain. In this SNiP, we highlight a study from Rué et al. that describes a bispecific nanobody construct that can bind an Alzheimer’s disease target as well as a receptor at the BBB interface to promote entry into the brain.
Media Adaptation for Suspension Cells
The success of an experiment can rely on the type of cell culture media used. In certain cases, you may need to adapt your current cell culture to a new medium. A stepwise process may be necessary to minimize stress and to retain normal cellular phenotype. Check out this Tip from the Bench to learn how to gradually adapt suspension cells to a new media formulation.