As biologists know, any and all components of an experiment can affect successful outcomes. Today we’ll discuss a sometimes overlooked variable of the transfection workflow: complex formation solution.
The complex formation solution serves as the vehicle where nucleic acids and TransIT® reagents coalesce into transfection complexes. This first step in any transfection application can set the stage for success of the downstream experiment. For Mirus reagents, there are a few important considerations to keep at top of mind for complexation to occur correctly.
Growth of Transfection Complexes in Various Complex Formation Media. Transfection complexes were formed in the indicated media. The size of the transfection complex was measured over time with DLS.
Mirus recommends forming transfection complexes in buffered, saline solutions such as Opti-MEM® or, in some cases, PBS or other serum-free basal media. Here is a list of our favorites:
NOTE: ‘complex formation solution’ and ‘cell culture media’ serve different purposes. The tips above apply to the complex formation solution, which is required solely for the unity of the nucleic acid and transfection reagent; therefore, the complex formation solution should not include additives that can interfere with this process. On the other hand, cell culture media, which provides the cells with the proper environment to support their health and growth can contain nutrients and additives such as serum and antibiotics. TransIT® Transfection Reagents are low toxicity, serum-compatible reagents, which eliminates the need for cell culture media changes.
Have a question about complex formation solutions or other transfection components? Let us know by email (email@example.com)!