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N to P Ratio

Cartoon of positive N divided by negative P.

In this TransMission Transfection 101, we bring you a brief primer on the ‘N to P ratio,’ which is a concept you may have encountered in the context of transfection. Here, we explain:

– What is the N to P ratio?
– How to calculate the N to P ratio
– Significance of the N to P ratio

Transfecting Insect Cells, Pt. 4

Howdy everyone! What we have all BEEn waiting for: the final installment of our ‘Transfecting Insect Cells’ series with Dr. Bees.

So far, we have discussed common insect cell lines and TransIT®-Insect (Part 1), producing baculovirus with flashBAC™ (Part 2) and key features of pOET Transfer Plasmids (Part 3). Today in Part 4, we will bug out on how baculovirus can be used to produce virus-like particles (VLPs) and other kinds of viral vectors, such as AAV!

Transfecting Insect Cells, Pt. 3

Howdy everyone! Dr. Bees here. So far, we have discussed common insect cell lines and the insect-specific transfection reagent TransIT®-Insect (Part 1) and producing baculovirus with flashBAC™ (Part 2). Today in Part 3, we will do a quick flyover of the pOET Transfer Plasmids, which complement flashBAC™ DNA like bees and honey!

Transfecting Insect Cells, Pt. 2

Howdy everyone! Dr. Bees is back with the 2nd installment of our insect transfection series, and bee-lieve me you will enjoy this one. Last time, we covered commonly transfected insect cell lines. Today we are going to focus on flashBAC™–what it is and how to use it for producing baculovirus.

Transfecting Insect Cells, Pt. 1

Howdy everyone! Guest writing for the TransMission is one of our field application specialists, Dr. Bees… which is a nom-de-plume, of course!

Starting the month of February for you love bugs and valentines, Dr. Bees will be doing a series of insect-themed posts, including transfection solutions that Mirus has developed for insect cells. In Part 1, we begin by introducing the cast of characters and the critical cell biology of insects.

Transfection Efficiency

“How successful was your transfection experiment?” Transfection efficiency is a measure used to answer this question. Because success can be defined in multiple ways, how to calculate transfection efficiency will depend on the type of transfection experiment.

In this TransMission Transfection 101 we will cover: the many meanings of transfection efficiency, common reporter assays to measure transfection efficiency, and how to improve transfection efficiency.

Understand Viral Titer & Quality

The cell and gene therapy field has burgeoned in the last decade with drug candidate treatments for many different diseases. Determining the correct patient dosage has become more important than ever to extend the reach of the multi-million-dollar manufacturing runs required to generate these viral therapies.

Genome copies (GC), capsids, GC/capsid ratios, percent full, functional titering… what does it all mean? In this Transfection 101, we summarize commonly used approaches to verify that manufactured viral vectors are of high quality and functionality, appropriate for use in patients.

Toxic Transfections

Why do cells die after transfection? How can you measure cytotoxicity? These questions answered in this TransMission Transfection 101, where we tackle cytotoxicity. Read on for advice on how to reduce transfection-related toxicity.

Deliverance: understanding transfection complexes

How do Mirus transfection reagents deliver? Today we’ll discuss what transfection complexes are and how nucleic acids are transfected into cells. This post will explain the purpose of steps in transfection protocols, including: transfection complex incubation, amount or ratio of reagent to nucleic acid to add, and what media to use for transfection complexes.