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The TransMission: Tips from the Bench

Storage of TransIT® Transfection Reagents & Kits

Last Updated: December 6, 2022

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Follow the proper storage and handling recommendations below to maintain optimal transfection performance of your TransIT® transfection reagents and kits.

Recommended Storage Temperature

The table below lists the recommended storage temperature for all TransIT® reagents and kits. This information as well as the product guarantee for each product can also be found on respective product pages and protocols here.


Product Storage Temperature 
TransIT®-293 Transfection Reagent     4°C 
TransIT®-2020 Transfection Reagent  -20°C
TransIT®-BrCa Transfection Reagent
TransIT®-CHO Transfection Kit   -20°C
TransIT-HeLaMONSTER® Transfection Kit   -20°C
TransIT®-Insect Transfection Reagent
TransIT®-Jurkat Transfection Reagent
TransIT®-Keratinocyte Transfection Reagent
TransIT®-Lenti Transfection Reagent
TransIT®-LT1 Transfection Reagent
TransIT®-mRNA Transfection Kit
TransIT®-Oligo Transfection Reagent
TransIT-PRO® Transfection Reagent and Kit
TransIT-siQUEST® Transfection Reagent
TransIT-TKO® Transfection Reagent
TransIT-VirusGEN® Transfection Reagent
TransIT-X2® Transfection Reagent  -20°C
VirusGEN® AAV Transfection Kit
Store TransIT-VirusGEN® at -20°C and AAV Complex Formation Solution and Enhancer at 4°C.
VirusGEN® LV Transfection Kit
Store TransIT-VirusGEN® at -20°C and LV Complex Formation Solution and LV Enhancer at 4°C.


Tips for Handling TransIT® Reagents

  • Store tightly capped at the recommended storage temperature when not in use.
  • Warm TransIT® reagents to room temperature before each use.
  • Gently vortex/mix reagents before each use.
  • Ensure any visible precipitates are fully dissolved before each use.

How to Dissolve Precipitates

Precipitates may occasionally form after storing TransIT® transfection reagents at 4°C or -20°C. Precipitation does not compromise product performance if they are completely dissolved. Precipitates are easily dissolved by gently warming--in your hands or briefly in a water bath--and with vortexing to mix. As a precaution, you can warm the reagents to room temperature and gently vortex the tube before each use to ensure uniformity of your reagent.




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