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The TransMission

Recent Posts

 

Which reagent should I use for transfecting oligos?

September 21, 2022

In today’s TransMission, we answer the FAQ: “Which reagent should I use for transfecting oligos?”

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SNiP-ing Success with CRISPR

September 6, 2022

It’s fitting that we SNiP (Small News in Pieces) an article about CRISPR. Read on if you think your CRISPR experiments are plagued by re-cutting, which can decrease gene-editing efficiency and introduce undesired mutations. Co-transfection with hideRNA may be a quick fix!

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Great Cycle Challenge

September 2, 2022

Special TransMission incoming from our GCC team leader (a.k.a. Director of Chemistry at Mirus Bio) Nick Rossi, Ph.D.!

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Optimizing siRNA Delivery

August 24, 2022

Because siRNA differs in size and structure from plasmid DNA, transfection reagents can be optimized and formulated differently for delivery of these RNA molecules. After selection of the appropriate transfection reagent, optimization of several experimental parameters is key to achieving the highest efficiency siRNA transfection and corresponding effective knockdown of target gene expression. Here we highlight our top "Tips from the Bench" for optimizing siRNA delivery. Note: most of these tips are applicable to delivery of other classes of oligos, including but not limited to miRNA and antisense oligonucleotides.

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Zap That!

August 8, 2022

We SNiP a study by Willerslev-Olsen et al., featuring Ingenio® electroporation of T cells with siRNA.

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Tell Me More About Telomeres

July 26, 2022

Today's TransMission is a SNiP (Small News in Pieces). Read how Jack et al. use TransIT® and Label IT® to study telomeres.

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Optimizing Plasmid DNA Delivery

July 13, 2022

Setting up successful transfection experiments for the first time can be a daunting task, especially when working with stubborn cells that are refractory to transfection. Variables such as cell line, passage number, confluency, DNA quality and even transfection reagent formulation all contribute to the likelihood of success (or failure) of a given transfection experiment. Here, we highlight our top "Tips from the Bench" for optimizing plasmid DNA delivery.

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Extending Viability in CHO Cells

June 30, 2022

A common question we encounter is: how can I extend CHO cell viability through the process of protein production? One common practice to maintain higher viability is to shift cell cultures to mild hypothermic conditions, e.g. 37°C to 32°C. Here, we discuss a hot (er… cold?) tip involving the CHOgro® High Yield Expression System, which also supports additional flexible protocol options for researchers who cannot shift cultures to 32°C or who desire extended cellular viability beyond one week following transfection.

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Deliverance: understanding transfection complexes

June 15, 2022

How do Mirus transfection reagents deliver? Today we'll discuss what transfection complexes are and how nucleic acids are transfected into cells. This post will explain the purpose of steps in transfection protocols, including: transfection complex incubation, amount or ratio of reagent to nucleic acid to add, and what media to use for transfection complexes.

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From AAV to TAZ

June 6, 2022

Today's TransMission is a SNiP (Small News in Pieces). Read how Hwang et al. use AAV to study mitochondrial biogenesis.

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(Re)Starting the Conversation

June 1, 2022

In this inaugural post, we introduce the goals of and Technical Support team behind the Mirus Bio Blog. A brief timeline of some milestones in Mirus history is also presented.

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