Mirus Bio receives ISO 13485:2016 certification, underscoring the quality of processes used to support GMP product portfolio. Read more

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Electroporation IRL

“Shock me like an electric eel.” To creatures sharing an ecosystem in the Amazon, co-existing with electric eels and their jolting aura is a lifelong experience. A group of researchers from Nagoya University explored whether shocks from electric eels could mediate nucleic acid delivery to zebrafish larvae! Could these eels be the living embodiment of the Ingenio® EZporator® Electroporation System?

Tune-In to the Immune System with Label IT®

Today’s TransMission explores an unconventional application of Mirus Bio’s Label IT® Nucleic Acid Labeling kits in engineering a new method of vaccine delivery, the microneedle array (MNA). MNAs are patches of micron-scale needles that delicately penetrate the skin in a painless manner, accessing an immune-rich population of cells. In this study, the authors fluorescently labeled immune-stimulating oligos using Label IT® Cy®3 and Label IT® Cy®5 to visualize their distribution on MNAs.

Read on to learn more about this unique method of stimulation!

Mixed Messages: a SNiP Double Feature

Cartoon of model organisms playing telephone with mRNA.

Transfection of three-dimensional cellular structures, like tissues and organs, and whole organisms can be tricky to accomplish. Today’s TransMission highlights not one but two SNiPs of studies featuring mRNA delivery to live planarian worms and rat fetuses. Read on to learn the methods employed by the authors of these studies to achieve this formidable feat!

What size are cell and gene therapies?

The relative size of AAV, lentivirus, transfection complex, and cell.

Have you ever given much thought to the relative size of the biological materials you work with every day in the lab? It can be mind-boggling to think about how something as tiny as a viral particle can have a life-saving impact for a patient with a debilitating genetic disease when delivered en masse.

But exactly how small of a package is all that life-changing power packed within? Test your knowledge in today’s blog post!

Behind the Scenes at St. Jude

Where do you go to find awesome transfection reagents, dedicated process development support, and a lot of snow? Answer: Mirus Bio!

At Mirus Bio, headquartered in Madison, Wisconsin, USA, you can find a little snow in the winter and a dedicated team of Transfection Experts at the ready to fix all your transfection troubles, no matter how large or small.

In this post, we highlight a recent podcast with Bryan Piras (Director of Manufacturing) at St. Jude Children’s Research Hospital, where he describes how their Process Development team was able to overcome challenges encountered during scale-up from an adherent to a suspension cell AAV8 production platform.

What’s in a (product) name?

What’s in a (product) name? That which we call a TransIT®
By any other name would deliver as efficient.

In today’s TransMission, we answer the FAQ: “Can I use this TransIT® to deliver that kind of nucleic acid?”

Understand Viral Titer & Quality

The cell and gene therapy field has burgeoned in the last decade with drug candidate treatments for many different diseases. Determining the correct patient dosage has become more important than ever to extend the reach of the multi-million-dollar manufacturing runs required to generate these viral therapies.

Genome copies (GC), capsids, GC/capsid ratios, percent full, functional titering… what does it all mean? In this Transfection 101, we summarize commonly used approaches to verify that manufactured viral vectors are of high quality and functionality, appropriate for use in patients.

Leveraging Avian AAV in Pekin Duck Research

Though typically seen in reference to human disease, AAV has applications apart from therapeutics for otherwise untreatable maladies. Today we SNiP a proof-of-concept study that features a unique use for TransIT-VirusGEN® in production of avian AAV for the transduction of Pekin duck cells in vitro and in vivo.