Stem Cell Transfection Solutions

Mirus Bio reagents enable high efficiency transfection of stem cells and other hard to transfect cell types used for stem cell research.

  • Perform genome editing - with TransIT-X2® Dynamic Delivery System 
  • Transfect DNA effectively - with TransIT®-2020 or TransIT®-LT1 Transfection Reagents
  • Perform repeated, low toxicity mRNA transfections - using TransIT®-mRNA Transfection Kit
  • Electroporate efficiently and cost-effectively - with Ingenio® Electroporation Solution

Poster presented at the Cellular Dynamics International User Group Meeting: High Efficiency Transfection of iCell® Cardiomyocytes and Stem Cell Relevant Cell Sources

Ideal Entry Points for Transfection in Stem Cell Workflow

Ideal Entry Points for Transfection in Stem Cell Workflow. Somatic cells such as adult fibroblast cells can be transfected or transduced via several methods (e.g. recombinant virus, plasmid, protein, mRNA, small molecule and miRNA) with a combination of transcription factors including KLF4, SOX2, c-Myc, Nanog, Oct-3/4 and LIN-28 to reprogram the cells to a pluripotent state. iPS cells can then be differentiated to a myriad of cell types through growth factor addition and/or transfection of selection markers driven by cell type specific promoters. Stem cell derived cell types such as cardiomyocytes, adipocytes, neural cells, pancreatic-β cells, and hematopoietic progenitor cells can provide researchers with relevant models for their experiments.

 

Genome Editing in Human IPSCs

CRISPR RNP Delivery Data iPSC

Efficient Genome Editing with Cas9 + Guide RNA Ribonucleoprotein Complexes. TransIT-X2® Dynamic Delivery System was used to deliver Cas9 protein/guide RNA ribonucleoprotein (RNP) complexes in human induced pluripotent stem cells (iPSCs). A T7E1 mismatch assay was used to measure cleavage efficiency at 48 hours post-transfection.

 

Primary and Stem Cells Transfected using Mirus Bio Reagents

Cell Type Nucleic Acid Mirus Reagent(s) Used Application Reference
Human embryonic stem cell derived neural progenitors Plasmid DNA TransIT®-2020 Stem cell transfection Scientist feedback
Human foreskin fibroblasts Plasmid DNA TransIT®-2020 DNA transfection Scientist feedback
Human induced pluripotent stem (iPS) cells Plasmid DNA TransIT®-2020 Stem cell transfection Data
  Plasmid DNA Ingenio® Stem cell transfection Data
  RNP TransIT-X2® Genome Editing Data
Human mesenchymal stem cells Plasmid DNA TransIT®-2020 Stem cell transfection Scientist feedback
Human skin fibroblasts Plasmid DNA TransIT®-2020 DNA transfection Scientist feedback
iCell® Cardiomyocytes Plasmid DNA TransIT®-LT1 Stem cell transfection Data
Mouse embryonic fibroblasts Plasmid DNA TransIT®-2020 DNA transfection Scientist feedback
Mouse embryonic stem cell derived cardiomyocytes Plasmid DNA TransIT®-2020 Stem cell transfection Scientist feedback
Normal human dermal fibroblasts (NHDF) Plasmid DNA TransIT®-2020 DNA transfection Scientist feedback
SRF -/- mouse embryonic stem cells Plasmid DNA TransIT®-LT1 Stem cell transfection Citation
BJ human neonatal foreskin fibroblasts mRNA TransIT®-mRNA Stem cell reprogramming Citation
C3H/10T1/2 mRNA TransIT®-mRNA Stem cell reprogramming Citation
CCD-1109Sk human normal adult skin fibroblasts mRNA TransIT®-mRNA Stem cell reprogramming Citation
MRC-5 human lung fibroblasts mRNA TransIT®-mRNA Stem cell reprogramming Citation
Primary human neonatal epidermal keratinocytes mRNA TransIT®-mRNA Stem cell reprogramming Citation
Primary human lung fibroblasts mRNA TransIT®-mRNA Stem cell reprogramming Citation
Human mesenchymal stem cells siRNA TransIT-TKO® Stem cell differentiation Citation
iCell® Cardiomyocytes siRNA TransIT-TKO® Knockdown of gene expression Data
Human adipose derived adult stem cells (hADAS) siRNA TransIT-siQUEST® Knockdown of gene expression Citation

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Citation Database: Check if our reagents have been used by other researchers to transfect your cell type
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