10X Better Performance Using 2.5X Less RNA. Huh7 cells were transfected with 2 µg of a yellow fever virus-luciferase expressing RNA replicon (YFV-luc) using the TransIT®-mRNA Transfection Kit or electroporated in parallel with 5 µg of the YFV-luc RNA replicon using an ECM630 Electroporator (BTX®) under optimal conditions. Twenty-four hours later, the cells were harvested and assayed for luciferase activity and total protein content. Luciferase activity was normalized to total protein content to correct for differences in cell number between samples (1).
(1) Gonzalez, G. et al. (2007) Selection of an Optimal RNA Transfection Reagent and Comparison to Electroporation for the Delivery of viral RNA. J. Virological. Methods. 145:14-21.
Simple Protocol Saves Over 4 Hours. Using TransIT®-mRNA saves more than four hours in comparison to electroporation.
Save Time and Valuable Resources. Simple comparison between TransIT®-mRNA and electroporation illustrates where you can save time, budget and materials.
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NEW! Transfection of Pseudouridine and 5-Methylcytidine Modified Messenger RNA for iPS Cell Reprogramming In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection. Jani, B., Fuchs, R. J. Vis. Exp. (61), e3702. Co-transfection of plasmid DNA and mRNA Stem Cell Applications TransIT®-mRNA for high-titer virus production Cell lines successfully transfected at Mirus using TransIT®-mRNA Transfection Kit