Applications: mRNA Transfection

Save Time and Resources over Electroporation

  • Superior Performance, Low Toxicity
  • Easier-to Use TransIT®-mRNA Kit Saves Valuable Time
  • No Electroporator needed - Saves Valuable Resources
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10X Better Performance Using 2.5X Less RNA

10X Better Performance Using 2.5X Less RNA. Huh7 cells were transfected with 2 µg of a yellow fever virus-luciferase expressing RNA replicon (YFV-luc) using the TransIT®-mRNA Transfection Kit or electroporated in parallel with 5 µg of the YFV-luc RNA replicon using an ECM630 Electroporator (BTX®) under optimal conditions. Twenty-four hours later, the cells were harvested and assayed for luciferase activity and total protein content. Luciferase activity was normalized to total protein content to correct for differences in cell number between samples (1).

(1) Gonzalez, G. et al. (2007) Selection of an Optimal RNA Transfection Reagent and Comparison to Electroporation for the Delivery of viral RNA. J. Virological. Methods. 145:14-21.

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Simple Protocol Saves Over 4 Hours; mRNA vs Electroporation

Simple Protocol Saves Over 4 Hours. Using TransIT®-mRNA saves more than four hours in comparison to electroporation.

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  TransIT®-mRNA Kit Electroporation
Cell Number ~3.8 x 105
(per well of 6-well plate)
Scalable based on culture vessel surface area
~5 x 106
(Plated in 6-well plate after electroporation)
RNA Amount 2.5 µg
(per well of 6-well plate)
Scalable based on culture vessel surface area
~5 µg
(per standard electroporation)
Serum Compatibility Compatible
- Enhances cell viability
- Simplifies protocol
- Requires serum free conditions
- Requires media changes

Save Time and Valuable Resources. Simple comparison between TransIT®-mRNA and electroporation illustrates where you can save time, budget and materials.