miRNA-enriched Samples From Mouse Brain and Heart Were Labeled With Cy®3 (brain) or Cy®5 (heart) Using the Label IT® miRNA Labeling Kit. The labeled samples were pooled and hybridized to duplicate miRMAX™ miRNA-specific microarrays (Rutgers). (A) Representative field from a hybridized array. (B) Average corrected Cy®3 (green bars, brain expression) and Cy®5 (red bars, heart expression) signals for 12 candidate miRNA features. (C) Predominant miRNA expression pattern determined by northern blotting (H = heart, B = brain) and published previously (Sempere et. al. 2004. Genome Biology 5(3): R13).
The Label IT® miRNA Labeling Reagents are Composed of a Fluorophore (or Biotin) Attached to the Positively Charged Label IT® Linker and Reactive Alkylating Group. The Label IT® Reagents covalently attach fluorophores (or biotin) to any reactive heteroatom in miRNAs without affecting downstream hybridization performance. miRNA labeling and purification can be completed in only two hours.