Applications: Low Toxicity Transfections

TransIT® Transfection Reagents Deliver to Multiple Cell Types with Minimal Toxicity

Four commonly used cell types: HepG2, THP-1, NT2 and HUVEC were analyzed for luciferase expression as well as cytotoxicity using a lactate dehydrogenase (LDH) assay for membrane leakage. At recommended doses, both TransIT®-LT1 and TransIT®-2020 display only minor cytotoxicity while delivering plasmid DNA with high efficiency into all four different cell lines tested. However, toxicity was most apparent, sometimes reading near 50% when using Lipofectamine® 2000 to deliver nucleic acid.

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