Low Toxicity in HeLa Cells

Mirus Broad Spectrum Transfection Reagents, TransIT®-LT1 and TransIT®-2020 Outperform Lipofectamine® 2000. HeLa cells were transfected with a GFP encoding plasmid DNA using either TransIT®-LT1, TransIT®-2020 or Lipofectamine® 2000 at reagent-to-DNA ratios of 3:1 for all reagents. Phase contrast and GFP images were taken in the same field of view at 8 and 24 hours post-transfection. Cells transfected with TransIT®-LT1, TransIT®-2020 remain healthy after 24 hours while maintaining high transfection efficiency, whereas cells transfected with Lipofectamine® 2000 display significant cytotoxicity after 24 hours.

TransIT® Broad Spectrum Reagents Balance High Efficiency Delivery with Low Toxicity. HeLa cells were transfected with luciferase encoding plasmid DNA using either TransIT®-LT1, TransIT®-2020 or Lipofectamine® 2000 for 24 hours. Transfection was measured by luciferase activity using a conventional assay. Cytotoxicity was assessed by quantifying the LDH released from the cytosol of damaged cells compared to cells alone. Higher transfection efficiency and lower cytotoxicity was observed in cells transfected with TransIT®-LT1 and TransIT®-2020 at optimal ratios than cells transfected with Lipofectamine® 2000.