Upgrade your virus production...
"Our lab tested TransIT®-LT1 from Mirus for virus production in 293T cells. At a 3:1 ratio using up to 5 µg of lentivirus, packaging and envelope plasmid, TransIT®-LT1 was far superior to Lipofectamine® in terms of viral titer downstream. So much so that our lab purchased the 0.4 ml product after using the trial."
-Andrew Paluch, University of Cincinnati
"Recently, Mirus Tech Support helped me troubleshoot moving my virus production to a serum free procedure. They helped me with many aspects of the procedure as I am new to this field. Most importantly, they alerted me that the media I was using for my transfections was recently identified by the R & D team at Mirus as not being appropriate for transfections. Once I changed transfection media, I was able to produce virus in my serum free system. Thank you to Mirus Tech Support. Your support is greatly appreciated."
- Ann Wrightstone, Sigma Aldrich
"Our lab successfully tested TransIT®-Insect Transfection Reagent for generating recombinant baculovirus in insect cells. Using TransIT®-Insect with multiple BEVS we were able to generate high-titer baculovirus that resulted in consistently higher protein expression in High Five™ and Sf9 cells compared to Cellfectin® II (Life Technologies)."
-Dr. Linda Lua (Director), The University of Queenland - Protein Expression Facility
"Our lab tested TransIT®-Insect for virus production in Sf9 cells. It performed better than Cellfectin® II that we currently use for transfecting these cells. Cytopathic effects (CPE) of the baculovirus were observed after 5 days with as little as 0.5 µg DNA per well of a 6-well plate. When using Cellfectin® and other reagents, we haven’t seen any results using the same amount of input DNA."
-Eric Carlin, Florida Gulf Coast University