Applications: High Titer Virus Production

Products for High Titer Virus Production

TransIT-Lenti Product Box Image

TransIT-VirusGEN® Transfection Reagent

TransIT-VirusGEN® Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent HEK 293T and suspension 293-F cell types to increase recombinant AAV production

  • Reliable and consistent high titer virus production
  • Scalability across different formats
  • Flexibility to address different virus and cell culture formats

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TransIT-Lenti Product Box Image

TransIT®-Lenti Transfection Reagent

TransIT®-Lenti Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent HEK 293T cell types to increase recombinant lentivirus production

  • Provide up to eight-fold higher functional titers
  • Simple protocol - no media change required, single harvest
  • Animal origin free

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TransIT-LT1 Transfection Reagent Product Image

TransIT®-LT1 Transfection Reagent

The first serum compatible, broad spectrum transfection reagent

  • Broad spectrum delivery to a variety of cell types
  • Low cellular toxicity
  • Deliver single or multiple plasmids

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TransIT-293 Transfection Reagent Product Image

TransIT®-293 Transfection Reagent

A high efficiency, low toxicity, DNA transfection reagent optimized for 293 cells

  • Maximize transfection performance in HEK 293 cells
  • Low cellular toxicity
  • Deliver single or multiple plasmids

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TransIT-2020 Product Box Image

TransIT®-2020 Transfection Reagent

TransIT®-2020 Transfection Reagent is a high performance, animal-free, broad spectrum DNA transfection reagent

  • Achieve high expression in many cell types, including hard to transfect cell lines and primary cells
  • Low cellular toxicity
  • Animal origin free

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TransIT-mRNA Transfection Reagent Product Image

TransIT®-mRNA Transfection Reagent

A high efficiency, low toxicity transfection reagent for large RNA

  • Generate high titers with superior RNA virus delivery
  • Produce virus more efficiently with less RNA than electroporation
  • Simple protocol with no media change required

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TransIT-Insect Transfection Reagent Product Image

TransIT®-Insect Transfection Reagent

Superior transient transfection for high yield baculovirus titers in insect cells

  • Exceptional DNA delivery in insect cell types including Sf9, High Five™ and S2
  • Ideal for baculovirus expression in insect cells
  • Non-liposomal, animal-origin free formulation

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Upgrade your virus production...

"Our lab tested TransIT®-LT1 from Mirus for virus production in 293T cells. At a 3:1 ratio using up to 5 µg of lentivirus, packaging and envelope plasmid, TransIT®-LT1 was far superior to Lipofectamine® in terms of viral titer downstream. So much so that our lab purchased the 0.4 ml product after using the trial."
-Andrew Paluch, University of Cincinnati

"Recently, Mirus Tech Support helped me troubleshoot moving my virus production to a serum free procedure. They helped me with many aspects of the procedure as I am new to this field. Most importantly, they alerted me that the media I was using for my transfections was recently identified by the R & D team at Mirus as not being appropriate for transfections. Once I changed transfection media, I was able to produce virus in my serum free system. Thank you to Mirus Tech Support. Your support is greatly appreciated."
- Ann Wrightstone, Sigma Aldrich

"Our lab successfully tested TransIT®-Insect Transfection Reagent for generating recombinant baculovirus in insect cells. Using TransIT®-Insect with multiple BEVS we were able to generate high-titer baculovirus that resulted in consistently higher protein expression in High Five™ and Sf9 cells compared to Cellfectin® II (Life Technologies)."
-Dr. Linda Lua (Director), The University of Queenland - Protein Expression Facility

"Our lab tested TransIT®-Insect for virus production in Sf9 cells. It performed better than Cellfectin® II that we currently use for transfecting these cells. Cytopathic effects (CPE) of the baculovirus were observed after 5 days with as little as 0.5 µg DNA per well of a 6-well plate. When using Cellfectin® and other reagents, we haven’t seen any results using the same amount of input DNA."
-Eric Carlin, Florida Gulf Coast University