TransIT® Transfection Reagents for RNA Mediated Virus Production

TransIT®-mRNA Transfection Kit Successfully Delivers Large Viral RNA. A 32 kb transcript of the murine coronavirus (MHV) was transfected into DBT cells using the TransIT®-mRNA Transfection Kit. Successful transfection was assessed 24-48 hours post-transfection by comparing a no RNA control (A) to the formation of syncytia (B) with MHV RNA. Syncytia were visualized by phase contrast microscopy.

Data courtesy of Mark Clementz, Loyola Univeristy of Chicago

TransIT®-mRNA Transfection Kit Delivers RNA More Efficiently than Electroporation. Huh7 cells were transfected with 2 µg of a yellow fever virus-luciferase expressing RNA replicon (YFV-luc) using the TransIT®-mRNA Transfection Kit or electroporation in PBS with 5 ug of the YFV-luc RNA replicon using an ECM630 Electroporator (BTX®) under optimal conditions. Twenty-four hours later, cells were harvested and assayed for luciferase activity and total protein content. Luciferase activity was normalized to total protein content to correct for differences in cell number between samples.