TransIT® Transfection Reagents for RNA Mediated Virus Production

  • Generate high titers with superior RNA virus delivery
  • Produce virus more efficiently with less RNA than electroporation
  • Save time with one simple protocol and no media change

RNA Mediated Virus Production Reagents

  • TransIT®-mRNA: generate high titers with superior RNA virus delivery
 

RNA Mediated Virus Production

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TransIT-mRNA Transfection Kit Successfully Delivers Large Viral RNA

TransIT®-mRNA Transfection Kit Successfully Delivers Large Viral RNA. A 32 kb transcript of the murine coronavirus (MHV) was transfected into DBT cells using the TransIT®-mRNA Transfection Kit. Successful transfection was assessed 24-48 hours post-transfection by comparing a no RNA control (A) to the formation of syncytia (B) with MHV RNA. Syncytia were visualized by phase contrast microscopy.

Data courtesy of Mark Clementz, Loyola Univeristy of Chicago

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TransIT-mRNA Transfection Kit Delivers RNA More Efficiently than Electroporation

TransIT®-mRNA Transfection Kit Delivers RNA More Efficiently than Electroporation. Huh7 cells were transfected with 2 µg of a yellow fever virus-luciferase expressing RNA replicon (YFV-luc) using the TransIT®-mRNA Transfection Kit or electroporation in PBS with 5 ug of the YFV-luc RNA replicon using an ECM630 Electroporator (BTX®) under optimal conditions. Twenty-four hours later, cells were harvested and assayed for luciferase activity and total protein content. Luciferase activity was normalized to total protein content to correct for differences in cell number between samples.

 

Citations for RNA mediated virus production using TransIT® Transfection Reagents

Virus Cell Type Product Citation
Picornavirus 293T/HeLa TransIT®-mRNA Greninger et al. J Virol 2012; 86: 3605-3616.
HCV HuH-7.5 TransIT®-mRNA Phan et al. J Virol; 2011; 85: 1193-1204.
Crimean-Congo Haemorrhagic Fever Virus BSR-T7/5 TransIT®-mRNA Bergeron et al. J Virol 2010; 84: 216-226.
HCV HuH-7 TransIT®-mRNA Jangra et al. J Virol 2010; 84: 6615-6625.
HEV PLC/PRF/5 TransIT®-mRNA Yamada et al. J Gen Virol 2009; 90: 457-462.

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Technical Resources

Mirus' Transfectopedia®: Why use recombinant viruses for nucleic acid delivery
Product Details: Find more information at the following product pages:

DNA Mediated Virus Production RNA Mediated Virus Production Baculovirus Production
TransIT-VirusGEN® Transfection Reagent
TransIT®-mRNA Transfection Kit TransIT®-Insect Transfection Reagent
TransIT®-Lenti Transfection Reagent
TransIT®-LT1 Transfection Reagent
TransIT®-293 Transfection Reagent
TransIT®-2020 Transfection Reagent

Reagent Agent Don't See Your Cell Type? Consult Reagent Agent® Transfection Database
Citation Database: Check if our reagents have been used by other researchers to transfect your cell type
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