TransIT® Transfection Reagents for DNA Mediated Virus Production

DNA Mediated Virus Production Reagents

  • TransIT-VirusGEN®: developed to provide high titer AAV & Lentivirus production with one reagent
  • TransIT®-Lenti: optimized for adherent Lentivirus production in 293T cells
  • TransIT®-LT1: the first serum compatible, broad spectrum transfection reagent
  • TransIT®-293: the first cell line specific reagent targeting efficient transfection of HEK-293 cells
  • TransIT®-2020: high efficiency broad spectrum, animal origin free transfection reagent
 

DNA Mediated Virus Production

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High Titer Virus Production Using TransIT-2020

Rescue of Influenza Virus by Reverse Transfection With TransIT®-2020 Transfection Reagent. 293T and MDBK cells are reverse transfected with up to 10 separate influenza virus rescue plasmids per experiment. Reagent:total DNA complexes are prepared with TransIT®-2020 Transfection Reagent at a 3:1 ratio of reagent to total DNA. Virus-containing supernatants are harvested 48-96 hr post-transfection. Titers from transfected cells can be as high as 107 pfu/ml. Rescued virus is then amplified on MDBK cells to increase titer and total yield. Viral stocks are subsequently titered by plaque assay on MDCK cells. Titers from representative rescue experiments with different viral strains and reassortants are shown. This experimental strategy is adapted from Neumann, et al. 2005 PNAS.

Data courtesy of Andrew Mehle, University of Wisconsin-Madison

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TransIT-293 Reagent Achieves High Transfection Efficiency in HEK 293 Cells

TransIT®-293 Reagent Achieves High Transfection Efficiency in HEK 293 Cells. HEK 293 cells were transfected with pEGFP using TransIT®-293 Reagent in complete media. Cells were visualized under fluorescent microscopy 24 hours post-transfection.

 

Citations for DNA mediated virus production using TransIT® Transfection Reagents

Virus Cell Type Product Citation
AAV
293-F TransIT-VirusGEN® New! 
293T TransIT-VirusGEN® New! 
Lentivirus 293-F TransIT-VirusGEN® New! 
293T TransIT®-Lenti New!
Retrovirus 293T TransIT®-LT1 Moon et al. Hum Mol Genet. 2014;23(2):449-66.
Phoenix Eco TransIT®-LT1 Saxena et al./em>. Mol Cell Bio 2009; 29: 2335-2345
293FT TransIT®-293 Haxhinasto et al./em>. J Exp Med 2008; 205: 565-574.
Reovirus BHK-T7 TransIT®-LT1 Stebbing et al./em>. J. Virol. 2014 vol. 88: 2572-2583.
BHK-T7 TransIT®-LT1 Doyle et al./em>. J Biol Chem 2012; 287: 8029-8038.
L929 TransIT®-LT1 Danthi et al./em>. J Virol 2008; 82: 161-172.
Influenza virus 293T/MDBK TransIT®-2020 Tran et al. J. Virol. 2013; 87: 13321-13329.
293T TransIT®-LT1 Imai et al./em>. PLoS Path 2010; 6: e1001106.
293T/MDCK TransIT®-LT1 Xing et al./em>. J Gen Viol 2009; 90: 1109-1118.
Chandipura virus BSRT-7/5 TransIT®-LT1 Stock et al. J Gen Virol 2014; 95: 38-43.
HPV Pseudovirus 293TT TransIT®-2020 Jagu et al. J. Virol. 2013; 87: 6127-6136.
LACV and JCV/LACV BHK TransIT®-LT1 Bennett et al. J Virol 2012; 86: 420-426.
Epstein-Barr 293 TransIT®-293 Hughes et al./em>. J Virol 2012; 86: 1034-1045.
HIV 293T/C-33A TransIT®-LT1 L’hernault et al./em>. J Virol 2012; 86: 5867-5876.
293T TransIT®-LT1 Galli et al./em>. J Virol 2010; 84: 7651-7661.
Linearised Polyomavirus MasPyV genome BHK and Vero TransIT®-2020 Orba et al. J Gen Virol 2011; 92 no. 4 789-795.
VLP 293T TransIT®-293 Crist et al./em>. J Virol 2009; 83: 2216-2225.
HTLV-1 293T TransIT®-LT1 Lamb et al./em>. JBC 2009; 284: 6575-6584.
SIV/SK 293T/MDCK TransIT®-LT1 Masic et al./em>. J Gen Virol 2009; 90: 375-385.
MLV 293T TransIT®-293 Rulli et al./em>. J Virol 2008; 82: 6566-6575.
 

Comparison of TransIT® Transfection Reagents for Virus Production

  TransIT-VirusGEN® TransIT®-Lenti TransIT®-LT1 TransIT®-293 TransIT®-2020 TransIT®-mRNA TransIT®-Insect
Transfect DNA or RNA DNA DNA DNA DNA DNA RNA DNA
Serum Compatible
Broad Spectrum Delivery - - - Insect cells only
Animal Origin Free - -
Citations New New

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Technical Resources

Mirus' Transfectopedia®: Why use recombinant viruses for nucleic acid delivery
Product Details: Find more information at the following product pages:

DNA Mediated Virus Production RNA Mediated Virus Production Baculovirus Production
TransIT-VirusGEN® Transfection Reagent
TransIT®-mRNA Transfection Kit TransIT®-Insect Transfection Reagent
TransIT®-Lenti Transfection Reagent
TransIT®-LT1 Transfection Reagent
TransIT®-293 Transfection Reagent
TransIT®-2020 Transfection Reagent

Reagent Agent Don't See Your Cell Type? Consult Reagent Agent® Transfection Database
Citation Database: Check if our reagents have been used by other researchers to transfect your cell type
Technical Support: Communicate directly with a transfection expert

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