TransIT®-mRNA Transfection Kit Successfully Delivers Large Viral RNA. A 32 kb transcript of the murine coronavirus (MHV) was transfected into DBT cells using the TransIT®-mRNA Transfection Kit. Successful transfection was assessed 24-48 hours post-transfection by comparing a no RNA control (A) to the formation of syncytia (B) with MHV RNA. Syncytia were visualized by phase contrast microscopy.
Data courtesy of Mark Clementz, Loyola Univeristy of Chicago