TransIT®-Insect for High Titer Baculovirus Production
Insect cell expression is a platform used to produce proteins with simple post-translational modifications. Transient transfection and recombinant baculovirus production are commonly used methods for insect cell expression. TransIT®-Insect Transfection Reagent is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types that offers:
- Exceptional DNA Delivery - In insect cell types including Sf9, High Five™ and S2
- High Baculovirus Production - Ideal for baculovirus expression in insect cells
- Serum Compatibility - Non-liposomal, animal-origin free formulation that eliminates media change
- Better Value - Low reagent amounts required per transfection
The buzz about TransIT®-Insect Reagent...
"Our lab successfully tested TransIT®-Insect Transfection Reagent for generating recombinant baculovirus in insect cells. Using TransIT®-Insect with multiple BEVS we were able to generate high-titer baculovirus that resulted in consistently higher protein expression in High Five™ and Sf9 cells compared to Cellfectin® II (Life Technologies)."
-Dr. Linda Lua (Director), The University of Queensland - Protein Expression Facility
"Our lab tested TransIT®-Insect for virus production in Sf9 cells. It performed better than Cellfectin® II that we currently use for transfecting these cells. Cytopathic effects (CPE) of the baculovirus were observed after 5 days with as little as 0.5 µg DNA per well of a 6-well plate. When using Cellfectin® and other reagents, we haven’t seen any results using the same amount of input DNA."
-Eric Carlin, Florida Gulf Coast University
Technical Resources
Product Details - Find more information at the following product pages:
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