The buzz about TransIT®-Insect Reagent...
"Our lab successfully tested TransIT®-Insect Transfection Reagent for generating recombinant baculovirus in insect cells. Using TransIT®-Insect with multiple BEVS we were able to generate high-titer baculovirus that resulted in consistently higher protein expression in High Five™ and Sf9 cells compared to Cellfectin® II (Life Technologies)."
-Dr. Linda Lua (Director), The University of Queensland - Protein Expression Facility
"Our lab tested TransIT®-Insect for virus production in Sf9 cells. It performed better than Cellfectin® II that we currently use for transfecting these cells. Cytopathic effects (CPE) of the baculovirus were observed after 5 days with as little as 0.5 µg DNA per well of a 6-well plate. When using Cellfectin® and other reagents, we haven’t seen any results using the same amount of input DNA."
-Eric Carlin, Florida Gulf Coast University