Applications: High Titer Virus Production

TransIT®-Insect for High Titer Baculovirus Production

Insect cell expression is a platform used to produce proteins with simple post-translational modifications. Transient transfection and recombinant baculovirus production are commonly used methods for insect cell expression. TransIT®-Insect Transfection Reagent is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types that offers:

  • Exceptional DNA Delivery - In insect cell types including Sf9, High Five™ and S2
  • High Baculovirus Production - Ideal for baculovirus expression in insect cells
  • Serum Compatibility - Non-liposomal, animal-origin free formulation that eliminates media change
  • Better Value - Low reagent amounts required per transfection
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Efficient Transfection of Baculovirus Genomic DNA Using TransIT-Insect Reagent

Efficient Transfection of Baculovirus Genomic DNA Using TransIT®-Insect Reagent. Transfections were performed in 6-well plates with 5 x 105 Sf9 cells per well using TransIT®-Insect Transfection Reagent  at the reagent-to-total DNA ratio of 3:1 (µl:µg). Cells were co-transfected with 0.5 µg of ProGreen™ baculovirus genomic vector DNA (AB Vector) encoding green-fluorescent protein (GFP) and 0.1 µg of pVL1393 transfer vector (AB Vector). (A) Fluorescence and phase contrast images were taken at 6 days post-transfection using a Zeiss S100 fluorescent microscope. Merge shown in (B).

The buzz about TransIT®-Insect Reagent...

"Our lab successfully tested TransIT®-Insect Transfection Reagent for generating recombinant baculovirus in insect cells. Using TransIT®-Insect with multiple BEVS we were able to generate high-titer baculovirus that resulted in consistently higher protein expression in High Five™ and Sf9 cells compared to Cellfectin® II (Life Technologies)."
-Dr. Linda Lua (Director), The University of Queensland - Protein Expression Facility

"Our lab tested TransIT®-Insect for virus production in Sf9 cells. It performed better than Cellfectin® II that we currently use for transfecting these cells. Cytopathic effects (CPE) of the baculovirus were observed after 5 days with as little as 0.5 µg DNA per well of a 6-well plate. When using Cellfectin® and other reagents, we haven’t seen any results using the same amount of input DNA."
-Eric Carlin, Florida Gulf Coast University

Technical Resources

Product Details - Find more information at the following product pages:

DNA Mediated Virus Production RNA Mediated Virus Production Baculovirus Production
TransIT-VirusGEN® Transfection Reagent
TransIT®-mRNA Transfection Kit TransIT®-Insect Transfection Reagent
TransIT®-Lenti Transfection Reagent
TransIT®-LT1 Transfection Reagent
TransIT®-293 Transfection Reagent
TransIT®-2020 Transfection Reagent

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