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Applications: High Throughput Transfection

HT Citations and Transfection Reagents

TransIT® Transfection Reagents have been used successfully by various researchers in high throughput applications in multi-well formats ranging from 96-well to 384-well (as mentioned in the Citations Table below).

Citations for Reverse Transfection using TransIT® Transfection Reagents

Citation Cell Type Transfected Nucleic Acid Transfected TransIT® Reagent Used Robotic system used Multi-well format Application
Wollenick et al. Nucleic Acids Res. 2012, 40 (5) 1928-1943 U2OS Plasmid DNA TransIT®-LT1 None 96-well Solid phase reverse transfection using a 96-well genome-wide full length cDNA array
Owens et al. J Biol Chem. 2010 February 26; 285(9): 6761-6769. HeLa Plasmid DNA TransIT®-LT1 Multidrop 384 (Titan) 384-well HT cell-based screens to detect stabilized protein targets following chemical mutagenesis
Zhao et al. Molecular Neurodegeneration 2009, 4:4 HeLa Plasmid DNA TransIT®-LT1 None 384-well HT fluorescence polarization-based Aβ degradation assay
Warzecha et al. Mol Cell. 2009 March 13; 33(5): 591-601. HEK 293T cell clone stably expressing the luciferasesplicing reporter Plasmid DNA TransIT®-293 Wellmate Handler (Matrix) 384-well HT cell-based genome-wide cDNA expression screening
Moser et al. PLoS ONE 2013 8(6):e68129 HeLa siRNA TransIT-TKO® Hydra DT robot 96-well HT genome-wide siRNA screen to identify host factors necessary for growth of the parasite Toxoplasma gondii
Lu et al. Oncogene. 2011 November 10; 30(45): 4567-4577. MDA-MB-468 siRNA TransIT-TKO® None 96-well HT kinome siRNA phosphoproteomic screen using an siRNA library targeting 541 kinases and kinase-related genes
Choudhary et al. J Biol Chem 2011 October, 286, 37187-37195. A549-Luc stable reporter cell line siRNA TransIT-siQUEST® Titertek, multidrop 384 cell dispenser 96-well HT siRNA screening of the human kinome
Andersen et al. Mol Ther. 2010 November; 18(11): 2018-2027. Human mesenchymal stem cells (hMSCs) siRNA TransIT-TKO® None Tissue culture plates coated by a lyophilization process with TransIT-TKO®/siRNA particles Tissue engineering using siRNA coated nanostructured scaffolds
Liu et al. J Biol Chem. 2008 August 22; 283(34) 23169-23178. Ikkγ-/- MEFs siRNA TransIT-siQUEST® None Not specified Mechanism study for RSV infection

All of the TransIT® transfection reagents can be used for reverse transfections with the following general recommendations based on the nucleic acid to be delivered. All of these are broad spectrum formulations are chemically distinct. Depending on the cell type being transfected, one reagent may have superior performance over others; for cell-type specific recommendations, please consult the Reagent Agent® transfection database.

Broad Spectrum HT Transfection Reagent Recommendations

Nucleic Acid to be Transfected HT Transfection Reagent Recommendation
Plasmid DNA and siRNA TransIT-X2® Dynamic Delivery System
Plasmid DNA including cDNA and shRNA encoding plasmid DNA libraries Option 1: TransIT-X2® Dynamic Delivery System
Option 2: TransIT®-2020 Transfection Reagent
Option 3: TransIT®-LT1 Transfection Reagent
siRNA/miRNA Option 1: TransIT-X2® Dynamic Delivery System
Option 2: TransIT-TKO® Transfection Reagent
Option 3: TransIT-siQUEST® Transfection Reagent
Large mRNA TransIT®-mRNA Transfection Kit
Oligonucleotides TransIT®-Oligo Transfection Reagent

Reagent Agent Don't See Your Cell Type? Consult Reagent Agent® Transfection Database
Citation Database: Check if our reagents have been used by other researchers to transfect your cell type
Technical Support: Speak directly with a transfection expert