HT Citations and Transfection Reagents
TransIT® Transfection Reagents have been used successfully by various researchers in high throughput applications in multi-well formats ranging from 96-well to 384-well (as mentioned in the Citations Table below).
Citations for Reverse Transfection using TransIT® Transfection Reagents
Citation |
Cell Type Transfected |
Nucleic Acid Transfected |
TransIT® Reagent Used |
Robotic system used |
Multi-well format |
Application |
Wollenick et al. Nucleic Acids Res. 2012, 40 (5) 1928-1943 |
U2OS |
Plasmid DNA |
TransIT®-LT1 |
None |
96-well |
Solid phase reverse transfection using a 96-well genome-wide full length cDNA array |
Owens et al. J Biol Chem. 2010 February 26; 285(9): 6761-6769. |
HeLa |
Plasmid DNA |
TransIT®-LT1 |
Multidrop 384 (Titan) |
384-well |
HT cell-based screens to detect stabilized protein targets following chemical mutagenesis |
Zhao et al. Molecular Neurodegeneration 2009, 4:4 |
HeLa |
Plasmid DNA |
TransIT®-LT1 |
None |
384-well |
HT fluorescence polarization-based Aβ degradation assay |
Warzecha et al. Mol Cell. 2009 March 13; 33(5): 591-601. |
HEK 293T cell clone stably expressing the luciferasesplicing reporter |
Plasmid DNA |
TransIT®-293 |
Wellmate Handler (Matrix) |
384-well |
HT cell-based genome-wide cDNA expression screening |
Moser et al. PLoS ONE 2013 8(6):e68129 |
HeLa |
siRNA |
TransIT-TKO® |
Hydra DT robot |
96-well |
HT genome-wide siRNA screen to identify host factors necessary for growth of the parasite Toxoplasma gondii |
Lu et al. Oncogene. 2011 November 10; 30(45): 4567-4577. |
MDA-MB-468 |
siRNA |
TransIT-TKO® |
None |
96-well |
HT kinome siRNA phosphoproteomic screen using an siRNA library targeting 541 kinases and kinase-related genes |
Choudhary et al. J Biol Chem 2011 October, 286, 37187-37195. |
A549-Luc stable reporter cell line |
siRNA |
TransIT-siQUEST® |
Titertek, multidrop 384 cell dispenser |
96-well |
HT siRNA screening of the human kinome |
Andersen et al. Mol Ther. 2010 November; 18(11): 2018-2027. |
Human mesenchymal stem cells (hMSCs) |
siRNA |
TransIT-TKO® |
None |
Tissue culture plates coated by a lyophilization process with TransIT-TKO®/siRNA particles |
Tissue engineering using siRNA coated nanostructured scaffolds |
Liu et al. J Biol Chem. 2008 August 22; 283(34) 23169-23178. |
Ikkγ-/- MEFs |
siRNA |
TransIT-siQUEST® |
None |
Not specified |
Mechanism study for RSV infection |
All of the TransIT® transfection reagents can be used for reverse transfections with the following general recommendations based on the nucleic acid to be delivered. All of these are broad spectrum formulations are chemically distinct. Depending on the cell type being transfected, one reagent may have superior performance over others; for cell-type specific recommendations, please consult the Reagent Agent® transfection database.
Broad Spectrum HT Transfection Reagent Recommendations
Don't See Your Cell Type? Consult Reagent Agent® Transfection Database
Citation Database: Check if our reagents have been used by other researchers to transfect your cell type
Technical Support: Speak directly with a transfection expert