High Throughput Transfection
Advances in high throughput (HT) screening as well as availability of multi-well cDNA/shRNA/siRNA/miRNA libraries have made high throughput transfection of different nucleic acids commonplace. High throughput transfections can be carried out in multi-well formats (such as 96-well, 384-well, etc.) using reverse transfection protocols that are amenable to automated robotic systems.
The most routinely employed transfection protocol where cells are seeded a day prior to transfection is referred to as "forward transfection". Forward transfection methods work well for most adherent cell types that are seeded a day prior to transfection in order to achieve an actively dividing cell population at the time of transfection. A typical forward transfection protocol using TransIT-X2® Dynamic Delivery System can be found here.
For high throughput applications, a "reverse transfection" protocol, where freshly passaged cells are added to pre-plated transfection complexes is ideal as it reduces overall experimental time for the end user. Cell culture time can be further reduced by using frozen assay-ready cells for some experiments (see example here). Reverse transfections are also compatible with most automated robotic systems.