Applications: Stem Cells
Products for Stem Cell Transfections
An advanced system for delivery of CRISPR/Cas9 components in human IPSCs
- Broad Spectrum Delivery – High efficiency delivery in a wide range of cell types
- Genome Editing – Deliver Cas9 protein/guide RNA ribonucleoprotein (RNP) complexes
- Technology – Novel, non-liposomal, polymeric delivery
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A high performance, animal-free, broad spectrum DNA transfection reagent
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
- Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
- Animal Origin Free – High performance with maximum compatibility
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A low toxicity, broad spectrum DNA transfection reagent
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
- Low Cellular Toxicity – Maintain cell density and reduce experimental biases
- High Efficiency Delivery – Achieve expression in a large population of cells
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A high efficiency, low toxicity transfection reagent for large RNA delivery
- Low Cellular Toxicity – Maintain cell density and reduce experimental biases
- High Efficiency Delivery – Achieve RNA delivery in a large population of cells
- Serum Compatible – Perform transfections in the presence of serum which eliminates the need for a media change
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High efficiency electroporation using any instrument
- High Efficiency Electroporation – Deliver to hard to transfect cell lines and primary cells
- Compatible with Most Conventional Electroporation Devices – Use your existing system including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®
- Save Money Without Sacrificing Performance – Available as a stand-alone solution or as part of a complete kit with cuvettes and cell droppers
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I recently tested TransIT®-2020 and TransIT®-LT1, and both reagents worked well in terms of their efficiency at transfecting human-derived iPS cells with CRISPR constructs and a fluorescent protein reporter. Through visual inspection, transfection efficiencies with TransIT®-2020 and TransIT®-LT1 were clearly higher than with Lipofectamine® 3000.
Fedir Kiskin
University of Cambridge
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