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Applications: Stem Cells

Products for Stem Cell Transfections

TransIT-2020 Product Box Image

TransIT-X2® Dynamic Delivery System

An advanced system for delivery of CRISPR/Cas9 components in human IPSCs

  • Broad Spectrum Delivery – High efficiency delivery in a wide range of cell types
  • Genome Editing – Deliver Cas9 protein/guide RNA ribonucleoprotein (RNP) complexes
  • Technology – Novel, non-liposomal, polymeric delivery

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TransIT-2020 Product Box Image

TransIT®-2020 Transfection Reagent

A high performance, animal-free, broad spectrum DNA transfection reagent

  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
  • Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
  • Animal Origin Free – High performance with maximum compatibility

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TransIT-2020 Product Box Image

TransIT®-LT1 Transfection Reagent

A low toxicity, broad spectrum DNA transfection reagent

  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • High Efficiency Delivery – Achieve expression in a large population of cells

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TransIT-mRNA Transfection Kit Product Image

TransIT®-mRNA Transfection Kit

A high efficiency, low toxicity transfection reagent for large RNA delivery

  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • High Efficiency Delivery – Achieve RNA delivery in a large population of cells
  • Serum Compatible – Perform transfections in the presence of serum which eliminates the need for a media change

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Ingenio Electroporation Solution Product Image

Ingenio® Electroporation Solution

High efficiency electroporation using any instrument

  • High Efficiency Electroporation – Deliver to hard to transfect cell lines and primary cells
  • Compatible with Most Conventional Electroporation Devices – Use your existing system including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®
  • Save Money Without Sacrificing Performance – Available as a stand-alone solution or as part of a complete kit with cuvettes and cell droppers

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I recently tested TransIT®-2020 and TransIT®-LT1, and both reagents worked well in terms of their efficiency at transfecting human-derived iPS cells with CRISPR constructs and a fluorescent protein reporter. Through visual inspection, transfection efficiencies with TransIT®-2020 and TransIT®-LT1 were clearly higher than with Lipofectamine® 3000.
Fedir Kiskin
University of Cambridge

Reagent Agent Don't See Your Cell Type? Consult Reagent Agent® Transfection Database
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