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Applications: Stem Cells

Stem Cell Electroporation using Ingenio® Kits

Electroporate Stem Cells Efficiently and Cost-effectively with Ingenio® Kits and Solution

  • High Efficiency - Electroporate hard to transfect cells including stem cells and their derivatives
  • Compatibility - Use with most conventional electroporation devices including Lonza-Amaxa® Nucleofector® II, Bio-Rad® or Harvard BTX®
  • Cost Savings - Reduce research budget while maximizing results

NEW PROTOCOL! Electroporation of Human Induced Pluripotent Stem (iPS) Cells with Ingenio® Electroporation Solution

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Plasmid DNA Electroporation of Human Induced Pluripotent Stem (iPS) Cells with Ingenio

Plasmid DNA Electroporation of Human Induced Pluripotent Stem (iPS) Cells with Ingenio®. The Ingenio® Electroporation Kit was used to transfect 2 x 106 iPS cells on the Amaxa® Nucleofector® II Device. Cells were electroporated with 8 µg ZsGreen expressing plasmid (Clontech) in 100 µl and plated in 6-well plates at 0.33 x 106 cells/well. Cells were visualized 24 hours post-transfection and imaged under 4X objective with an Olympus IX71® Inverted Microscope. Images are (A) phase contrast, and (B) green fluorescence. Cells were also assayed 24 hours post-transfection on an Accuri® Cytometer. The histogram (C) shows unelectroporated cells (black line) compared to cells electroporated with plasmid using the Ingenio® Electroporation Kit (green line).

Data courtesy of
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The Ingenio® Electroporation Kit is routinely used in our lab to transfect induced pluripotent stem cells (iPSCs) via electroporation and yields extremely high transfection efficiency. The Ingenio® Kit is entirely compatible with the Amaxa® Nucleofector® and is also a much more cost effective solution.
Elizabeth Dominguez
Cellular Dynamics International (CDI)
 

Ingenio® Kits provide a Cost-Effective and Stable Alternative to Nucleofector® Kitsa

Product Shelf Life Cost/Electroporationb Savings
Amaxa® Nucleofector® Kit V (VCA-1003) 3 months $14.64 -
Ingenio® Electroporation Kit (MIR 50112) 1 year $8.68 41%

a Compatible with Amaxa® Nucleofector® II device
b Based on 2014 U.S. List prices from company websites and protocol recommendations (25 RXN kit).


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