Applications: Stem Cells
DNA Transfection Into Stem Cells and Their Derivatives
TransIT®-2020 and TransIT®-LT1 Transfection Reagents for DNA Delivery
- High Efficiency Delivery - Achieve high expression in many cell types, including hard to transfect cell types such as stem cells and primary cells
- Low Cellular Toxicity - Maintain cell density and reduce experimental biases
- Suitable for Many Applications - Perform gene expression studies, promoter analysis and stable cell line generation
Download Protocol: Reverse Transfection of Human Induced Pluripotent Stem (iPS) Cells with TransIT®-LT1 Transfection Reagent
I recently tested TransIT®-2020 and TransIT®-LT1, and both reagents worked well in terms of their efficiency at transfecting human-derived iPS cells with CRISPR constructs and a fluorescent protein reporter. Through visual inspection, transfection efficiencies with TransIT®-2020 and TransIT®-LT1 were clearly higher than with Lipofectamine® 3000.
Fedir Kiskin
University of Cambridge
Primary and Stem Cells Transfected using Mirus Bio Reagents
Cell Type |
Nucleic Acid |
Mirus Reagent(s) Used |
Application |
Reference |
Human embryonic stem cell derived neural progenitors |
Plasmid DNA |
TransIT®-2020 |
Stem cell transfection |
Scientist feedback |
Human foreskin fibroblasts |
Plasmid DNA |
TransIT®-2020 |
DNA transfection |
Scientist feedback |
Human induced pluripotent stem (iPS) cells |
Plasmid DNA |
TransIT®-2020 |
Stem cell transfection |
Data |
|
Plasmid DNA |
Ingenio® |
Stem cell transfection |
Data |
Human mesenchymal stem cells |
Plasmid DNA |
TransIT®-2020 |
Stem cell transfection |
Scientist feedback |
Human skin fibroblasts |
Plasmid DNA |
TransIT®-2020 |
DNA transfection |
Scientist feedback |
iCell® Cardiomyocytes |
Plasmid DNA |
TransIT®-LT1 |
Stem cell transfection |
Data |
Mouse embryonic fibroblasts |
Plasmid DNA |
TransIT®-2020 |
DNA transfection |
Scientist feedback |
Mouse embryonic stem cell derived cardiomyocytes |
Plasmid DNA |
TransIT®-2020 |
Stem cell transfection |
Scientist feedback |
Normal human dermal fibroblasts (NHDF) |
Plasmid DNA |
TransIT®-2020 |
DNA transfection |
Scientist feedback |
SRF -/- mouse embryonic stem cells |
Plasmid DNA |
TransIT®-LT1 |
Stem cell transfection |
Citation |
Don't See Your Cell Type? Consult Reagent Agent® Transfection Database
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