Applications: Stem Cells
Stem Cell Transfection Solutions
Mirus Bio reagents enable high efficiency transfection of stem cells and other hard to transfect cell types used for stem cell research.
- Perform genome editing - with TransIT-X2® Dynamic Delivery System
- Transfect DNA effectively - with TransIT®-2020 or TransIT®-LT1 Transfection Reagents
- Perform repeated, low toxicity mRNA transfections - using TransIT®-mRNA Transfection Kit
- Electroporate efficiently and cost-effectively - with Ingenio® Electroporation Solution
Poster presented at the Cellular Dynamics International User Group Meeting: High Efficiency Transfection of iCell® Cardiomyocytes and Stem Cell Relevant Cell Sources
Genome Editing in Human IPSCs
Primary and Stem Cells Transfected using Mirus Bio Reagents
Cell Type |
Nucleic Acid |
Mirus Reagent(s) Used |
Application |
Reference |
Human embryonic stem cell derived neural progenitors |
Plasmid DNA |
TransIT®-2020 |
Stem cell transfection |
Scientist feedback |
Human foreskin fibroblasts |
Plasmid DNA |
TransIT®-2020 |
DNA transfection |
Scientist feedback |
Human induced pluripotent stem (iPS) cells |
Plasmid DNA |
TransIT®-2020 |
Stem cell transfection |
Data |
|
Plasmid DNA |
Ingenio® |
Stem cell transfection |
Data |
|
RNP |
TransIT-X2® |
Genome Editing |
Data |
Human mesenchymal stem cells |
Plasmid DNA |
TransIT®-2020 |
Stem cell transfection |
Scientist feedback |
Human skin fibroblasts |
Plasmid DNA |
TransIT®-2020 |
DNA transfection |
Scientist feedback |
iCell® Cardiomyocytes |
Plasmid DNA |
TransIT®-LT1 |
Stem cell transfection |
Data |
Mouse embryonic fibroblasts |
Plasmid DNA |
TransIT®-2020 |
DNA transfection |
Scientist feedback |
Mouse embryonic stem cell derived cardiomyocytes |
Plasmid DNA |
TransIT®-2020 |
Stem cell transfection |
Scientist feedback |
Normal human dermal fibroblasts (NHDF) |
Plasmid DNA |
TransIT®-2020 |
DNA transfection |
Scientist feedback |
SRF -/- mouse embryonic stem cells |
Plasmid DNA |
TransIT®-LT1 |
Stem cell transfection |
Citation |
BJ human neonatal foreskin fibroblasts |
mRNA |
TransIT®-mRNA |
Stem cell reprogramming |
Citation |
C3H/10T1/2 |
mRNA |
TransIT®-mRNA |
Stem cell reprogramming |
Citation |
CCD-1109Sk human normal adult skin fibroblasts |
mRNA |
TransIT®-mRNA |
Stem cell reprogramming |
Citation |
MRC-5 human lung fibroblasts |
mRNA |
TransIT®-mRNA |
Stem cell reprogramming |
Citation |
Primary human neonatal epidermal keratinocytes |
mRNA |
TransIT®-mRNA |
Stem cell reprogramming |
Citation |
Primary human lung fibroblasts |
mRNA |
TransIT®-mRNA |
Stem cell reprogramming |
Citation |
Human mesenchymal stem cells |
siRNA |
TransIT-TKO® |
Stem cell differentiation |
Citation |
iCell® Cardiomyocytes |
siRNA |
TransIT-TKO® |
Knockdown of gene expression |
Data
|
Human adipose derived adult stem cells (hADAS) |
siRNA |
TransIT-siQUEST® |
Knockdown of gene expression |
Citation |
Don't See Your Cell Type? Consult Reagent Agent® Transfection Database
Citation Database: Check if our reagents have been used by other researchers to transfect your cell type
Technical Support: Speak directly with a transfection expert