CRISPR RNP Delivery with Ingenio® Electroporation Solution. Ribonucleoprotein (RNP) complexes targeting (A) PPIB or (B) WTAP were electroporated into K562 and Jurkat cells. The RNP complex, composed of 750 nM Cas9 protein (EnGen Cas9 NLS, New England Biolabs) and 1500 nM pre-complexed two-part gRNA (IDT), was electroporated using the Ingenio® Electroporation Solution (Mirus Bio) and a Gene Pulser Xcell™ Eukaryotic System (Bio-Rad® Laboratories). Exponential pulse conditions of 130V (A) & 150V (B), 950 µF for K562 and 150V, 950 µF for Jurkat cells were applied to triplicate 0.2 cm cuvettes, 100 µl volume, 10 x 106 cells/ml +/- RNP complex. A T7E1 mismatch assay was used to measure cleavage efficiency at 48 hours post-transfection. Non-specific bands (NSP) were observed in the negative control of both cell lines. Cleavage efficiency was calculated based on the ratio of cleaved band intensities to the sum of cleaved and uncleaved band intensities minus the average signal of the non-specific band(s) in negative control lanes.