Same Results, Lower Cost Using Ingenio® with Amaxa® Nucleofector® II

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Similar Performance on Amaxa Nucleofector Device

Ingenio® Solution Provides Comparable Efficiency on Amaxa® Nucleofector®. Cells were electroporated in parallel with an EGFP reporter vector using a Nucleofector® II Device (Amaxa®) and either the Ingenio® Electroporation Solution or the Nucleofector® Kit V (Amaxa®) according to Amaxa®’s recommended protocol for each cell line [HL-60 (T-019), Jurkat E6-1(X-001), K-562 (T-016), THP-1 (V-001), SK-N-MC (A-023), and MCF-7 (P020)]. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population.

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Ingenio and Gene Pulser Electroporator Yield Comparable Performance to Amaxa Nucleofector System

Ingenio® Solution Shows Similar Results with Amaxa® Nucleofector® and Other Electroporators. Ingenio® Solution used with Gene Pulser Electroporator Yields Comparable High Efficiency to the Amaxa® Nucleofector® II System: HL-60, Jurkat E6-1, K562, THP-1 and SK-N-MC cells were electroporated with the same EGFP reporter on the same day, using either Ingenio® Electroporation Solution in the Gene Pulser XCell (Bio-Rad®) (red bars) or the Amaxa® Solution V in Amaxa® Nucleofector® II (Lonza) (light grey bars).

High Electroporation Efficiency using Ingenio® Solution

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Ingenio Provides Both Superior Efficiency and Cell Viability Compared to Other Solutions

Ingenio® Provides Both Superior Efficiency and Cell Viability Compared to Other Solutions. Cells were electroporated in parallel with an EGFP reporter vector using either Ingenio® Electroporation Solution, PBS or the Gene Pulser Electroporation Buffer (Bio-Rad®) on the Gene Pulser Xcell™ Eukaryotic System. (A) EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. (B) Cells were assayed for viablility by propidium iodide staining and flow cytometry analysis. Experiments were performed in triplicate on three separate days and the data averaged.

Ingenio® Kits provide a Cost-Effective and Stable Alternative to Nucleofector® Kitsa

Product Shelf Life Cost/Electroporationb Savings
Amaxa® Nucleofector® Kit V (VCA-1003) 3 months $14.64 -
Ingenio® Electroporation Kit (MIR 50112) 1 year $8.68 41%

a Compatible with Amaxa® Nucleofector® II device
b Based on 2014 U.S. List prices from company websites and protocol recommendations (25 RXN kit).


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Technical Resources

Reagent Agent Don't See Your Cell Type? Consult Reagent Agent® Transfection Database
Citation Database: Check if Ingenio® has been used by other researchers to transfect your cell type
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Ingenio® Product Webpage: View more product details

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