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Applications: Electroporation with Ingenio®

Ingenio® Electroporation Solution and EZporator® Electroporation System

The Best Choice for High Efficiency Electroporation

  • High Efficiency Electroporation of Hard to Transfect Cells - Including primary cells and stem cells
  • Compatible with Most Conventional Electroporation Devices - Including the Ingenio® EZporator®, Lonza-Amaxa®, Bio-Rad® and Harvard BTX®
  • Cost Savings Using the Ingenio® Electroporation Kits - On your existing Amaxa® Nucleofector® without sacrificing performance
  • Long Shelf Life - One year shelf life allows maximum usage for your experiments
  • Buy Only What You Need - Available as solution alone or as part of a kit with cuvettes and cell droppers

Mirus Bio offers a full range of electroporation offerings, including; Ingenio® Electroporation Solution, electroporation cuvettes, and the new Ingenio® EZporator® Electroporation System. These products are designed to high efficiency delivery to hard-to-transfect cells without the cost or complication of other systems.  

The Ingenio® Electroporation Kit is routinely used in our lab to transfect induced pluripotent stem cells (iPSCs) via electroporation and yields extremely high transfection efficiency. The Ingenio® Kit is entirely compatible with the Amaxa® Nucleofector® and is also a much more cost effective solution.
Elizabeth Dominguez
Cellular Dynamics International (CDI)
 
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Efficient Broad Spectrum Plasmid DNA Delivery Using Ingenio Solution with Amaxa Nucleofector II

Efficient Broad Spectrum Plasmid DNA Delivery Using Ingenio® Solution with Amaxa® Nucleofector® II. Ingenio® Electroporation Kits were used to transfect indicated cell types using Amaxa® Nucleofector® II Device. Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population. See the recommended experimental conditions.

Ingenio® Kits Provide a Cost-Effective and Stable Alternative to Nucleofector® Kitsα

Product Shelf Life Cost/Electroporationβ Savings
Amaxa® Nucleofector® Kit V (VCA-1003) 3 Months $15.20 -
Ingenio® Electroporation Kit (MIR 50112) 1 Year $9.16 40%

α Compatible with Amaxa® Nucleofector® II device
βBased on 2016 U.S. list prices from company websites and protocol recommendations (25 RXN kit)

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CRISPR RNP Delivery with Ingenio Electroporation Solution Targeting PPIB

CRISPR RNP Delivery with Ingenio® Electroporation Solution. K562 and Jurkat cells were electroporated with a Cas9 protein/gRNA, ribonucleoprotein (RNP) complex, composed of 750 nM Cas9 protein (EnGen® Cas9 NLS, NEB) and 1500 nM pre-complexed two-part gRNA (IDT) targeting PPIB using the Ingenio® Electroporation Solution and a Gene Pulser Xcell™ Eukaryotic System. Exponential pulse conditions of 130V, 950 µF for K562 and 150V, 950 µF for Jurkat cells were applied to triplicate 0.2 cm cuvettes, 100 µl volume, 10 x 106 cells/ml +/- RNP complex. A T7E1 mismatch assay was used to measure cleavage efficiency at 48 hours post-transfection. Non-specific bands (NSP) were observed in the negative control of both cell lines. Cleavage efficiency was calculated based on the ratio of cleaved band intensities to the sum of cleaved and uncleaved band intensities minus the average signal of the non-specific band(s) in negative control lanes.

Technical Resources

Reagent Agent Don't See Your Cell Type? Consult Reagent Agent® Transfection Database
Citation Database: Check if Ingenio® has been used by other researchers to transfect your cell type
Technical Support: Speak directly with a transfection expert
Ingenio® Product Webpage: View more product details