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Diifferent TransIT® Transfection Reagents are recommended depending on the combinations of nucleic acids being delivered. All of the following reagent recommendations are of broad spectrum formulations that are chemically distinct. Depending on the cell type being transfected, one reagent may have superior performance over others; for cell-type specific recommendations, please consult the Reagent Agent® transfection database or contact techsupport@mirusbio.com

Nucleic Acid(s) to be Co-transfected Transfection Reagent Free Sample
Multiple plasmid DNAs Option 1: TransIT-X2® Dynamic Delivery System
Option 2: TransIT®-2020 Transfection Reagent
Option 3: TransIT®-LT1 Transfection Reagent
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Multiple siRNAs Option 1: TransIT-X2® Dynamic Delivery System
Option 2: TransIT-TKO® Transfection Reagent
Option 3: TransIT-siQUEST® Transfection Reagent
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Plasmid DNA and siRNA TransIT-X2® Dynamic Delivery System Request sample
Multiple mRNAs TransIT®-mRNA Transfection Kit Request sample
Plasmid DNA and mRNA TransIT-X2® Dynamic Delivery System and
TransIT®-mRNA Transfection Kit
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Multiple oligonucleotides TransIT®-Oligo Transfection Reagent Request sample
Plasmid DNA and oligonucleotides TransIT-X2® Dynamic Delivery System and
TransIT®-Oligo Transfection Reagent
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Other combinations Contact techsupport@mirusbio.com  

If a single transfection reagent is not suitable for a specific co-delivery application, then separate transfection complexes can be prepared using the optimal transfection reagent for each nucleic acid and added to the cells at the same time. As long as the individual transfection protocols are compatible, this method would ensure uptake of all nucleic acids by the cell simultaneously. An example is the co-delivery of plasmid DNA and mRNA that is explained in detail here.

 

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