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Diifferent TransIT® Transfection Reagents are recommended depending on the combinations of nucleic acids being delivered. All of the following reagent recommendations are of broad spectrum formulations that are chemically distinct. Depending on the cell type being transfected, one reagent may have superior performance over others; for cell-type specific recommendations, please consult the Reagent Agent® transfection database or contact email@example.com
If a single transfection reagent is not suitable for a specific co-delivery application, then separate transfection complexes can be prepared using the optimal transfection reagent for each nucleic acid and added to the cells at the same time. As long as the individual transfection protocols are compatible, this method would ensure uptake of all nucleic acids by the cell simultaneously. An example is the co-delivery of plasmid DNA and mRNA that is explained in detail here.