Increase Expression of Secreted Proteins and Antibodies in 293 Cells
- Better or Equal Expression than 293fectin™ Transfection Reagent
- Express Secreted Proteins and Antibodies at High Levels
High Levels of Antibody Expression at Different Volume Scales using TransIT-PRO® Transfection Kit
| Antibody Expression |
Scale(L) |
Yield(mg/L) |
Eight different antibodies were transiently expressed in FreeStyle™ 293‐F cells (Life Technologies) using the TransIT‐PRO Transfection Reagent (1:1) according to manufacturer’s instructions. Cells were grown in FreeStyle™ 293 Expression Medium and transfected at a density of 1 x 106 cells/ml.
The scale of the transfection for each protein varied between 1 ‐ 11 L of culture.
The protein yields range from 11.2‐ 33.3 mg/L.
Data courtesy of a TransIT‐PRO customer from a Contract Research Organization (CRO).
More details here. |
1 |
18.1 |
| 5 |
15.4 |
| 6 |
25.0 |
| 6 |
11.2 |
| 6 |
20.0 |
| 5 |
18.8 |
| 10 |
13.3 |
| 11 |
33.3 |
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