Increase Expression of Secreted Proteins and Antibodies in 293 Cells

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  • Express Secreted Proteins and Antibodies at High Levels
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Achieve High Protein Yields in Suspension 293 Cells

Achieve High Protein Yields Using TransIT-PRO® Transfection Kit in Suspension 293 Cells. Ten different secreted (non‐antibody) proteins were transiently expressed in FreeStyle™ 293‐F cells (Life Technologies) using TransIT‐PRO (1.5:1) or 293fectin™ (Life Technologies, 2:1) transfection reagents according to manufacturer's protocol. Cells were grown in FreeStyle™ 293 Expression Medium at transfected at a density of 1 x 106 cells/ml. The scale of the transfection for each protein varied between 1‐6 L of culture. More experimental details here.

Data courtesy of a TransIT‐PRO pharmaceutical customer.

 

High Levels of Antibody Expression at Different Volume Scales using TransIT-PRO® Transfection Kit

Antibody Expression Scale(L) Yield(mg/L)
Eight different antibodies were transiently expressed in FreeStyle™ 293‐F cells (Life Technologies) using the TransIT‐PRO Transfection Reagent (1:1) according to manufacturer’s instructions. Cells were grown in FreeStyle™ 293 Expression Medium and transfected at a density of 1 x 106 cells/ml.

The scale of the transfection for each protein varied between 1 ‐ 11 L of culture.
The protein yields range from 11.2‐ 33.3 mg/L.

Data courtesy of a TransIT‐PRO customer from a Contract Research Organization (CRO).
More details here.
1 18.1
5 15.4
6 25.0
6 11.2
6 20.0
5 18.8
10 13.3
11 33.3

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