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Applications | GMP Transfection for AAV Production

The Best Choice for High Efficiency Electroporation

Mirus Bio offers a full range of electroporation offerings, including; Ingenio® Electroporation Solutionelectroporation cuvettes, and the new Ingenio® EZporator® Electroporation System. These products are designed to high efficiency delivery to hard-to-transfect cells without the cost or complication of other systems.

  • High Efficiency Electroporation of Hard to Transfect Cells – Including primary cells and stem cells
  • Compatible with Most Conventional Electroporation Devices – Including the Ingenio® EZporator®, Lonza-Amaxa®, Bio-Rad® and Harvard BTX®
  • Cost Savings Using the Ingenio® Electroporation Kits – On your existing Amaxa® Nucleofector® without sacrificing performance
  • Long Shelf Life – One year shelf life allows maximum usage for your experiments
  • Buy Only What You Need – Available as solution alone or as part of a kit with cuvettes and cell droppers

 

Same Results, Lower Cost Using Ingenio® with Amaxa® Nucleofector® II


Ingenio® Solution Provides Comparable Efficiency on Amaxa® Nucleofector®. Cells were electroporated in parallel with an EGFP reporter vector using a Nucleofector® II Device (Amaxa®) and either the Ingenio® Electroporation Solution or the Nucleofector® Kit V (Amaxa®) according to Amaxa®’s recommended protocol for each cell line [HL-60 (T-019), Jurkat E6-1(X-001), K-562 (T-016), THP-1 (V-001), SK-N-MC (A-023), and MCF-7 (P020)]. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population.


Ingenio® Solution Shows Similar Results with Amaxa® Nucleofector® and Other Electroporators. Ingenio® Solution used with Gene Pulser Electroporator Yields Comparable High Efficiency to the Amaxa® Nucleofector® II System: HL-60, Jurkat E6-1, K562, THP-1 and SK-N-MC cells were electroporated with the same EGFP reporter on the same day, using either Ingenio® Electroporation Solution in the Gene Pulser XCell (Bio-Rad®) (red bars) or the Amaxa® Solution V in Amaxa® Nucleofector® II (Lonza) (light grey bars).


High Electroporation Efficiency using Ingenio® Solution

Ingenio® Provides Both Superior Efficiency and Cell Viability Compared to Other Solutions. Cells were electroporated in parallel with an EGFP reporter vector using either Ingenio® Electroporation Solution, PBS or the Gene Pulser Electroporation Buffer (Bio-Rad®) on the Gene Pulser Xcell™ Eukaryotic System. (A) EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. (B) Cells were assayed for viablility by propidium iodide staining and flow cytometry analysis. Experiments were performed in triplicate on three separate days and the data averaged.


Ingenio® Kits provide a Cost-Effective and Stable Alternative to Nucleofector® Kitsa

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"The Ingenio® Electroporation Kit is routinely used in our lab to transfect induced pluripotent stem cells (iPSCs) via electroporation and yields extremely high transfection efficiency. The Ingenio® Kit is entirely compatible with the Amaxa® Nucleofector® and is also a much more cost effective solution."
Elizabeth Dominguez
Cellular Dynamics International (CDI)

Efficient Broad Spectrum Plasmid DNA Delivery Using Ingenio® Solution with Amaxa® Nucleofector® II. 
Ingenio® Electroporation Kits were used to transfect indicated cell types using Amaxa® Nucleofector® II Device. Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population. See the recommended experimental conditions (PDF).

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