The success of an experiment can rely heavily on the type of cell culture media used. Many choices are available and selecting the right media can ensure the optimal performance of your cells. In certain cases, you may need to adapt your current cell culture to a new medium. Transferring the cells directly into a new medium in one step may be possible, but a stepwise process may be necessary to minimize stress and to retain normal cellular phenotype. To adapt suspension CHO and 293 cultures, The Transfection Experts at Mirus Bio use the following protocol. The general procedure also works well for adapting other cell types to new media formulations.
Stepwise Adaptation of Suspension Cell Culture to a New Medium. To gradually condition cells to growing in a new medium, they are sub-cultured in an increasing ratio of the new target medium relative to the current medium. If cells are not doubling as expected, and/or viability is < 95%, do not increase the percentage of the target medium in the culture. Cell doubling time should be normal and viability over 95% before each stepwise increase. After successful adaptation in 100% target medium, cells may be cryogenically stored in the target medium; henceforth, they can be cultured in the target medium immediately out of thaw.
Depending on your current and target suspension cell media, it may be possible to simply use the new medium during routine maintenance with no special considerations. If the cells do not readily adapt to your new suspension cell medium, try the stepwise adaptation protocol below: