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Search Results for: TransIT-LT1
Applications | Low Toxicity Transfections
Applications | Low Toxicity Transfections REQUEST AN EVALUATION Low Toxicity Transfection Balance Expression Toxicity Toxicity and Stress Response Low Tox Across Cell Types Products High
Applications | High Titer Virus Production
Applications | High Titer Virus Production REQUEST AN EVALUATION DNA Mediated Virus Production Lentivirus Production RNA Mediated Virus Production Baculovirus Production Products for Virus Production
Applications | Stable Cell Line Generation
Applications | Stable Cell Line Generation Overview Step 1: Antibiotic Kill Curve Step 2: Stable Transfection Step 3: Selection/Expansion Products Stable Cell Line Generation Most
Applications | Stem Cell Transfection
Applications | Stem Cell Transfection Stem Cell Solutions DNA Transfection RNA Transfection Electroporation Products Stem Cell Transfection Solutions Mirus Bio reagents enable high efficiency transfection
Applications | Co-transfection
Co-transfection or co-delivery of multiple nucleic acids – either similar or different in nature is a technique routinely employed by cell biologists to address increasingly complex experimental design and applications.
Applications | RNAi Gene Silencing
Applications | RNAi Gene Silencing TransIT for Gene Silencing siRNA Mediated Pathway miRNA Mediated Pathway shRNA Mediated Pathway Products TransIT® Transfection Reagents for Gene Silencing
Enrich CRISPR’d Cells with Label IT®
Today’s TransMission is a SNiP of one of our favorite papers featuring Label IT®. Nasri and Mir et al. describe an elegant use for Label IT® in enriching CRISPR/Cas-edited cells. For use in the clinic, it is highly desirable to limit the number of nonedited cells that may compete with the therapeutic, modified cells. Read on to learn how Label IT® was used to enhance the CRISPR genome-editing workflow!
Discovery Research
Discovery Research Spend less time searching for a transfection reagent and more time moving your innovations forward. We offer a range of effective transfection solutions
CRISPR Prime Editors Unleashed
“United we stand, divided we… small?”
CRISPR prime editing, introduced in 2019, harnesses a Frankensteinian enzyme–a Cas nickase fused to a reverse transcriptase–to perform gene edits with putatively higher fidelity than traditional CRISPR/Cas genome editing systems. In this SNiP, we highlight recent work from Grünewald et al. that shows the prime editor fusion can be split without negative consequence to editing, suggesting that the nickase and reverse transcriptase modules operate in trans. This finding is a boon for delivery and use of prime editing machinery with space-constrained vectors, such as AAV and lentiviral vectors.
Read on to learn more about prime editing and the authors’ discovery.