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Search Results for: TransIT-LT1

Applications | Co-transfection

Co-transfection or co-delivery of multiple nucleic acids – either similar or different in nature is a technique routinely employed by cell biologists to address increasingly complex experimental design and applications.

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Icon showing cells sorted with Label IT and Cas9 RNP.

Enrich CRISPR’d Cells with Label IT®

Today’s TransMission is a SNiP of one of our favorite papers featuring Label IT®. Nasri and Mir et al. describe an elegant use for Label IT® in enriching CRISPR/Cas-edited cells. For use in the clinic, it is highly desirable to limit the number of nonedited cells that may compete with the therapeutic, modified cells. Read on to learn how Label IT® was used to enhance the CRISPR genome-editing workflow!

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Discovery Research

Discovery Research Spend less time searching for a transfection reagent and more time moving your innovations forward. We offer a range of effective transfection solutions

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CRISPR Prime Editors Unleashed

“United we stand, divided we… small?”

CRISPR prime editing, introduced in 2019, harnesses a Frankensteinian enzyme–a Cas nickase fused to a reverse transcriptase–to perform gene edits with putatively higher fidelity than traditional CRISPR/Cas genome editing systems. In this SNiP, we highlight recent work from Grünewald et al. that shows the prime editor fusion can be split without negative consequence to editing, suggesting that the nickase and reverse transcriptase modules operate in trans. This finding is a boon for delivery and use of prime editing machinery with space-constrained vectors, such as AAV and lentiviral vectors.

Read on to learn more about prime editing and the authors’ discovery.

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Chromothripsis and the Epstein-Barr Virus

Chromothripsis is a term used to describe chromosome shattering. In this SNiP, we highlight a recent study by Li et al. that revealed a link between Epstein-Barr Virus (EBV) infection and chromosomal instability. The authors identify a locus on chromosome 11q23 that is susceptible to binding by multiple copies of the EBV protein EBNA1. They propose a mechanism by which this concentrated binding and subsequent chromosomal breakage could explain the association between EBV and a variety of cancers.

And, did we mention that >90% of adults worldwide live with EBV? Read on to learn more.

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