Regulate pLIVE® Expression Level

pLIVE® is a vector system designed for long-term expression in the livers of laboratory rodents. In this Tips from the Bench, we share how gene expression levels can be modulated by changing the relative position of the gene of interest within the pLIVE® multiple cloning site. We also discuss testing the pLIVE® construct in vitro before beginning in vivo studies.