Adapt Adherent CHO Cells to Suspension Culture
Chinese Hamster Ovary (CHO) cells are a popular workhorse for production of recombinant therapeutic proteins. Amenable to both adherent and suspension culture, CHO cells offer versatility in growth platform that is favorable to the end user’s requirements.
In this Tips from the Bench, we highlight factors that differentiate adherent and suspension CHO cell cultures. Also, we describe a simple protocol for transitioning adherent CHO cells to suspension growth (and serum-free medium, if required).