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Optimizing Plasmid DNA Delivery

Setting up successful transfection experiments for the first time can be a daunting task, especially when working with stubborn cells that are refractory to transfection. Variables such as cell line, passage number, confluency, DNA quality and even transfection reagent formulation all contribute to the likelihood of success (or failure) of a given transfection experiment. Here, we highlight our top “Tips from the Bench” for optimizing plasmid DNA delivery.