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TransIT® Transfection Reagents for High-titer Virus Production

  • Produce virus confidently with validated Mirus products
    • TransIT®-2020, TransIT®-LT1, and TransIT®-293 for recombinant DNA virus production
    • TransIT®-mRNA for recombinant RNA virus production
  • Increase virus titers with high efficiency, low toxicity transfection reagents
  • Decrease production time by using lower quantities of nucleic acids and serum compatible reagents
 
Table 1. Recent Citations for DNA and RNA mediated virus production using TransIT® Transfection Reagents
Virus Cell Type Product Citation
HCV HuH-7.5 TransIT-mRNA Phan et al. J Virol 2011; 85: 1193-1204.
CCHFV BSR-T7/5 TransIT-mRNA Bergeron et al. J Virol 2010; 84: 216-226.
HIV-1 293T TransIT-LT1 Galli et al. J Virol 2010; 84: 7651-7661.
Influenza H5N1 293T TransIT-LT1 Imai et al. PLoS Path 2010; 6: e1001106.
HCV HuH-7 TransIT-mRNA Jangra et al. J Virol 2010; 84: 6615-6625.
Lentivirus 293T TransIT-293 Kato et al. J Biochem 2010; 147: 63-71.
VLP 293T TransIT-293 Crist et al. J Virol 2009; 83: 2216-2225.
HTLV-1 293T TransIT-LT1 Lamb et al. JBC 2009; 284: 6575-6584.
SIV/SK 293T/MDCK TransIT-LT1 Masic et al. J Gen Virol 2009; 90: 375-385.
Retrovirus Phoenix Eco TransTI-LT1 Saxena et al. Mol Cell Bio 2009; 29: 2335-2345.
Influenza H9N2 293T/MDCK TransIT-LT1 Xing et al. J Gen Virol 2009; 90: 1109-1118.
HEV PLC/PFR/5 TransIT-mRNA Yamada et al. J Gen Virol 2009; 90: 457-462.
Influenza H5N1 293T TransIT-LT1 Yen et al. PNAS 2009; 106: 286-291.
 
Table 2. Comparison of TransIT® Transfection Reagents
  TransIT-2020 TransIT-LT1 TransIT-293 TransIT-mRNA
Transfect DNA or RNA DNA DNA DNA RNA
High Efficiency
Low Cell Toxicity
Serum Compatible
Easy-to-Use
Broad Spectrum Delivery -
Animal Origin Free - -
Citations New
 
T1F2. Serum Compatible
Figure 1. TransIT Transfection Reagents are Serum Compatible.
All TransIT Transfection Reagents are low toxicity and do not require a media change. Save time and money by adding formed complexes directly to cells in media containing serum and avoiding unnecessary media changes.
 

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