The TransIT®-LT1 Protocol Results in Greater Gene Expression
The TransIT®-LT1 and FuGENE® 6 Protocols Recommend Different
Reagent and DNA Amounts
Recommended Starting Conditions
(per well of a 6-well plate)
Final Reagent to DNA
Ratio (µl:µg) |
3:1 |
1.5:1 |
6:1 |
| |
| TransIT-LT1 Reagent |
7.5 µl |
|
|
| DNA |
2.5 µg |
|
|
| |
| FuGENE 6 Reagent |
3 µl |
3 µl |
6 µl |
| DNA |
1 µg |
2 µg |
1 µg |
2.5X More DNA and Reagent Results in up to 12X More Gene Expression
The indicated cell lines were transfected in duplicate with the recommended amounts of transfection reagent and luciferase expression vector (per 12-well) from the TransIT-LT1 and FuGENE 6 protocols to achieve a final 3:1 ratio. Cells were harvested 24 hours post-transfection and assayed for luciferase activity.
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