The Proof is in the Performance
Comparable Luciferase Expression In Side-by-Side Transfections
The indicated cell lines were transfected in duplicate with 1 µg of a luciferase expression vector per well of a 12-well plate using either 3 µl of the TransIT®-LT1 or FuGENE® 6 Reagents. Cells were harvested 24 hours post-transfection and assayed for luciferase activity.
The TransIT®-LT1 Protocol Results in Greater Gene Expression
The TransIT®-LT1 and FuGENE® 6 Protocols Recommend Different
Reagent and DNA Amounts
Recommended Starting Conditions
(per well of a 6-well plate)
Final Reagent to DNA
Ratio (µl:µg) |
3:1 |
1.5:1 |
6:1 |
| |
| TransIT-LT1 Reagent |
7.5 µl |
|
|
| DNA |
2.5 µg |
|
|
| |
| FuGENE 6 Reagent |
3 µl |
3 µl |
6 µl |
| DNA |
1 µg |
2 µg |
1 µg |
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