siXpress® PCR Vector Systems
siXpress® PCR Vector Systems
| Product No. | Quantity | Price | Add to |
| siXpress® Human U6 PCR Vector System |
| MIR 7300 | 20 reactions | $482.00 |
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| siXpress® Human H1 PCR Vector System |
| MIR 7301 | 20 reactions | $482.00 |
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| siXpress® Mouse U6 PCR Vector System |
| MIR 7302 | 20 reactions | $482.00 |
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siXpress® PCR Systems Separate Components
- Enables efficient in situ expression of siRNA and knockdown.
- Synthesize multiple siRNA expression cassettes by PCR in hours.
- Select the siRNA expression cassette that provides the most desirable effect.
- Option of cloning PCR generated expression cassette in supplied vector.
- Complete Systems: provided with primers, template, controls and TransIT®-LT1 Transfection Reagent.
Data
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| Figure 1. CHO-luc cells (CHO-K1 cells expressing a stably integrated firefly luciferase), were transfected with siXpress® PCR siRNA expression cassettes using TransIT®-LT1 Transfection Reagent. Three siXpress® promoter vectors were tested: human U6, mouse U6, and human H1. An untargeted SEAP (secreted alkaline phosphatase) hairpin siRNA expression cassette (blue), a luciferase specific hairpin siRNA expression cassette (orange), and a luciferase specific hairpin siRNA control cassette (yellow) were amplified for each promoter. The luciferase specific hairpin siRNA control cassette was amplified directly from the corresponding luciferase hairpin siRNA expression plasmid. 500 ng of each cassette were transfected with TransIT®-LT1 Transfection Reagent and assayed after 48 hours. Luciferase siXpress® cassettes and luciferase control cassettes were normalized to the SEAP cassettes for each corresponding promoter. |
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