TransIT®-siPAK and TransIT®-siPAK Plus Trial Kits

Figure 1. Inhibition of Stably Expressed Firefly Luciferase Using the TransIT-siQUEST or TransIT–TKO Reagent. Cell lines stably expressing firefly luciferase were transfected with an anti-firefly luciferase siRNA or the reagent alone controls using either the TransIT-siQUEST or TransIT-TKO Reagent. Twenty-four hours post-transfection, luciferase expression was measured and compared to the reagent alone controls.

Figure 2. Comparison of TransIT-siQUEST and TransIT-TKO Reagents to a Competitor Reagent. Reporter plasmid DNA expressing firefly and sea pansy luciferase were co-transfected into the indicated cell line using the TransIT-LT1® Reagent. Firefly luciferase was knocked down by transfection of 25nM anti-firefly luciferase siRNA using either TransIT-siQUEST (orange), TransIT-TKO (blue) or Competitor L2 (gray) Reagents. Bars indicate the percent of normalized firefly luciferase expression as compared to the reagent alone control 24 hours post-transfection.

Figure 3. The Label IT® RNAi Delivery Controls Allow Quick Assessment of Delivery Efficiency For In Vitro Applications. HeLa cells were transfected in serum-containing media with the Label IT Fluorescein RNAi Delivery Control (green) using the TransIT-TKO Transfection Reagent. Twenty-four hours post-transfection, the cells were fixed, then counterstained to locate the nuclei (blue) and the actin (red).