TransIT®-siPAK and TransIT®-siPAK Plus Trial Kits
A trial pack containing two high efficiency, low toxicity siRNA transfection reagents for mammalian cells
| Product No. | Quantity | Price | Add to |
| TransIT®-siPAK Kit |
| MIR 2260 | 0.1 ml each TransIT-siQUEST and TransIT-TKO Reagents | $112.00 |
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| TransIT®-siPAK Plus Kit |
| MIR 2270 | 0.1 ml each TransIT-siQUEST and TransIT-TKO Reagents and 10 µg Label IT RNAi Delivery Control, Fluorescein | $167.00 |
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Resources
Protocol
MSDS
Contact Technical Support
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- High Knockdown Efficiency—Achieve optimal gene silencing in a large percentage of cells to ensure experimental success.
- Low Cellular Toxicity—Maintain cell density and reduce experimental biases.
- Reproducible Results—Obtain consistent, targeted gene knockdowns from day to day.
- Trial Size—Identify the best siRNA transfection reagent for your cells and applications.
Cell Types Successfully Transfected by Mirus Bio:
A549, BHK-21, BNL.CL2, C2C12, C6, CHO-KI, COS-7, Daoy, DB-TRG-05MG, D1-TNC1, DU145, HEK 293, HeLa, Hepa1c1c7, HepG2, human astrocytes, Jurkat, Keratinocytes (NIKS), MCF-7, Neuro-2a, NIH 3T3, PC-3, primary mouse hepatocytes, RAW 264.7, SK-N-MC, THP-1, Vero.
Data
Figure 1: Inhibition of Stably Expressed Firefly Luciferase Using the TransIT-siQUEST or TransIT–TKO Reagent.
Figure 2: Comparison of TransIT-siQUEST and TransIT-TKO Reagents to a Competitor Reagent.
Figure 3: The Label IT® RNAi Delivery Controls Allow Quick Assessment of Delivery Efficiency.
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| Figure 1. Inhibition of Stably Expressed Firefly Luciferase Using the TransIT-siQUEST or TransIT–TKO Reagent. Cell lines stably expressing firefly luciferase were transfected with an anti-firefly luciferase siRNA or the reagent alone controls using either the TransIT-siQUEST or TransIT-TKO Reagent. Twenty-four hours post-transfection, luciferase expression was measured and compared to the reagent alone controls. |
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| Figure 2. Comparison of TransIT-siQUEST and TransIT-TKO Reagents to a Competitor Reagent. Reporter plasmid DNA expressing firefly and sea pansy luciferase were co-transfected into the indicated cell line using the TransIT-LT1® Reagent. Firefly luciferase was knocked down by transfection of 25nM anti-firefly luciferase siRNA using either TransIT-siQUEST (orange), TransIT-TKO (blue) or Competitor L2 (gray) Reagents. Bars indicate the percent of normalized firefly luciferase expression as compared to the reagent alone control 24 hours post-transfection. |
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Figure 3. The Label IT® RNAi Delivery Controls Allow Quick Assessment of Delivery Efficiency For In Vitro Applications. HeLa cells were transfected in serum-containing media with the Label IT Fluorescein RNAi Delivery Control (green) using the TransIT-TKO Transfection Reagent. Twenty-four hours post-transfection, the cells were fixed, then counterstained to locate the nuclei (blue) and the actin (red). |
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