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pLIVE® In Vivo Expression and Reporter Vectors

A vector system designed to achieve long term expression in the livers of laboratory mice

Product No. Quantity Price Add to

pLIVE™ Vector

MIR 5420

20 µg

$351.00

pLIVE™ Vector Complete System

MIR 5320

20 µg of each (all 3 vectors)

$568.00

pLIVE™ Vector/LacZ Control Vector Kit

MIR 5520

20 µg of each vector

$459.00

pLIVE™ Vector/SEAP Control Vector Kit

MIR 5620

20 µg of each vector

$459.00

To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.

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Sustained Gene Expression - Achieve long term liver gene expression in the mouse liver through 8 months post-injection.
Versatile Platform - Available with positive control vectors expressing either LacZ or SEAP.

Maps

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Sequences

The pLIVE Vector Download PDF	*The pLIVE-LacZ Vector* Download PDF	The pLIVE-SEAP Vector Download PDF

The pLIVE Vectors are covered by patents pending of Mirus Bio LLC. Purchase of this product conveys a license for use of this product only for research by the purchaser in not-for-profit institutions. The purchaser may only transfer vectors modified with sequences for protein expression to researchers in not-for-profit institutions who agree to be bound by this license. This license does not include the right to perform research at or on behalf of a for-profit entity, the right to transfer unmodified vectors to others or the right to use the product for any commercial purpose (e.g. therapeutics, manufacturing, diagnostics or sale or fee-for-service use of animal models). Prospective users at for-profit institutions or those who wish to perform research on behalf of for-profit institutions or any other commercial purpose must contact Mirus Bio LLC for a commercial license. If you require a commercial license to use this product and you decide not to take a license, please return the unopened product to Mirus Bio LLC for a full refund.

Data

Figure 1: The Promoter in the pLIVE Vector Outperforms the CMV Promoter for Long-term Liver Expression.
Figure 2: Long-term Expression of Human Placental Secreted Alkaline Phosphatase (SEAP) in Mice after Hydrodynamic Delivery of the pLIVE-SEAP Vector.
Figure 3: Strong Liver Expression of ß-galactosidase from the pLIVE-LacZ Vector after Hydrodynamic Tail Vein Injection.

Figure 1. The Promoter in the pLIVE Vector Outperforms the CMV Promoter for Long-term Liver Expression. The pLIVE-SEAP Vector (20 µg) and pCMV-SEAP (10 µg, CMV promoter-driven SEAP vector) were each delivered to four ICR mice using the hydrodynamic tail vein injection procedure and the TransIT-QR Hydrodynamic Delivery Solution (MIR 5240). At the indicated days post-injection, serum from each mouse was collected and the level of SEAP activity present was determined using the Phospha-Light™ SEAP Assay Kit (Applied Biosystems). The average SEAP activity from each group is presented.

Figure 2. Long-term Expression of Human Placental Secreted Alkaline Phosphatase (SEAP) in Mice after Hydrodynamic Delivery of the pLIVE-SEAP Vector. The pLIVE-SEAP Vector was delivered to three C57Bl/6 mice using the hydrodynamic tail vein injection procedure and the TransIT-QR Hydrodynamic Delivery Solution (MIR 5240). At the indicated times post-injection, serum from each mouse was collected and the level of SEAP activity was determined using the Phospha-Light™ SEAP Assay Kit (Applied Biosystems). Average SEAP activity at each time point is presented.

A	B

Figure 3. Strong Liver Expression of ß-galactosidase from the pLIVE-LacZ Vector after Hydrodynamic Tail Vein Injection. The pLIVE-LacZ Vector (Panel A) was delivered to an ICR mouse using the hydrodynamic tail vein injection procedure and the TransIT-QR Hydrodynamic Delivery Solution (MIR 5240). At 24 hours post-injection, the liver was harvested, sectioned and stained with X-gal using the Beta-Gal Staining Kit (MIR 2600) to demonstrate ß-galactosidase activity (blue cells). The cells were then counterstained with hematoxylin and eosin Y to stain the nuclei and cytoplasms, respectively. The control mouse (LacZ negative) in Panel B was stained in parallel to Panel A and contains no detectable ß-galactosidase activity.