TransIT®-mRNA Transfection Kit

Figure 1. The TransIT-mRNA Transfection Kit Efficiently Delivers LacZ mRNA to CHO-K1 Cells. Using the TransIT-mRNA Transfection Kit, CHO-K1 cells were mock transfected (A) or transfected with a capped and polyadenylated LacZ encoding mRNA (B). Approximately 18 hrs post-transfection the cells were stained using Mirus Bio’s Beta-gal Staining Kit to identify the LacZ transfected cells.

Figure 2. The TransIT-mRNA Transfection Kit Efficiently Delivers mRNA to a Variety of Cell Lines. Using the TransIT-mRNA Transfection Kit, cells were transfected with a capped and polyadenylated EGFP encoding mRNA. Approximately 18 hrs post-transfection the cells were analyzed by flow cytometry to identify the EGFP expressing cells.

Figure 3. High Level Luciferase Expression after Delivery of a Luciferase mRNA using the TransIT-mRNA Transfection Kit. Cells in 12-well plates were transfected with a capped and polyadenylated mRNA encoding luciferase using the TransIT-mRNA Transfection Kit. Approximately 18 hrs post-transfection the cells were harvested and the total luciferase activity per well was determined.

Figure 4. Achieve Superior mRNA Delivery Performance with the TransIT-mRNA Transfection Kit. The indicated cell lines were transfected at approximately 80% confluency with a capped and polyadenylated mRNA encoding firefly luciferase using either the TransIT-mRNA Transfection Kit or Competitor Reagent Q. Approximately 18 hours post-transfection, the cells were harvested, lysed and the amount of luciferase activity present in each well was determined. The results were normalized to the activity of the TransIT-mRNA Kit transfected samples (set as 100%). The black diamonds illustrate the average percent cell confluency compared to the TransIT-mRNA transfected wells for each cell line demonstrating the toxicity of Reagent Q. In each experiment, the cells transfected with the TransIT-mRNA Transfection Kit were approximately 100% confluent at the time of harvest.