TransIT®-mRNA Transfection Kit
A high efficiency, low toxicity, large RNA transfection reagent for mammalian cells
| Product No. | Quantity | Price | Add to |
| MIR 2225 | 0.25 ml | $280.00 |
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| MIR 2250 | 0.5 ml | $503.00 |
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| MIR 2255 | 5 x 0.5 ml | $2,257.00 |
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| MIR 2256 | 10 x 0.5 ml | $4,262.00 |
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Each Kit is supplied with the TransIT-mRNA Transfection Reagent and the mRNA Boost Reagent.
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Electroporation Comparison Data
TransMessenger™ Comparison Data
- Low Cellular Toxicity—Maintain cell density and reduce experimental biases.
- High Efficiency Delivery—Achieve RNA delivery in a large population of cells to ensure experimental success.
- Serum Compatible—Perform transfections in the presence of serum which eliminates the need for a media change and maintains cellular health.
- Deliver Various Sizes of RNA—Ideal for specialized applications, such as viral production and protein expression from mRNA.
Cell lines successfully transfected at Mirus Bio:
A549, CHO-K1, COS-7, HEK 293, HeLa, HepG2, NIH 3T3, and Vero cell lines.
Data
Figure 1: The TransIT-mRNA Transfection Kit Efficiently Delivers the lacZ mRNA to CHO-K1 Cells.
Figure 2: The TransIT-mRNA Transfection Kit Efficiently Delivers mRNA to a Variety of Cell Lines.
Figure 3: High Level Luciferase Expression after Delivery of a Luciferase mRNA using the TransIT-mRNA Transfection Kit.
Figure 4: Achieve Superior mRNA Delivery Performance with the TransIT-mRNA Transfection Kit.
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| A. Mock Transfection Control |
B. lacZ mRNA Transfection |
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| Figure 1. The TransIT-mRNA Transfection Kit Efficiently Delivers lacZ mRNA to CHO-K1 Cells. Using the TransIT-mRNA Transfection Kit, CHO-K1 cells were mock transfected (A) or transfected with a capped and polyadenylated lacZ encoding mRNA (B). Approximately 18 hrs post-transfection the cells were stained using Mirus Bio’s Beta-gal Staining Kit to identify the lacZ transfected cells. |
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| Figure 2. The TransIT-mRNA Transfection Kit Efficiently Delivers mRNA to a Variety of Cell Lines. Using the TransIT-mRNA Transfection Kit, cells were transfected with a capped and polyadenylated EGFP encoding mRNA. Approximately 18 hrs post-transfection the cells were analyzed by flow cytometry to identify the EGFP expressing cells. |
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| Figure 3. High Level Luciferase Expression after Delivery of a Luciferase mRNA using the TransIT-mRNA Transfection Kit. Cells in 12-well plates were transfected with a capped and polyadenylated mRNA encoding luciferase using the TransIT-mRNA Transfection Kit. Approximately 18 hrs post-transfection the cells were harvested and the total luciferase activity per well was determined. |
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| Figure 4. Achieve Superior mRNA Delivery Performance with the TransIT-mRNA Transfection Kit. The indicated cell lines were transfected at approximately 80% confluency with a capped and polyadenylated mRNA encoding firefly luciferase using either the TransIT-mRNA Transfection Kit or Competitor Reagent Q. Approximately 18 hours post-transfection, the cells were harvested, lysed and the amount of luciferase activity present in each well was determined. The results were normalized to the activity of the TransIT-mRNA Kit transfected samples (set as 100%). The black diamonds illustrate the average percent cell confluency compared to the TransIT-mRNA transfected wells for each cell line demonstrating the toxicity of Reagent Q. In each experiment, the cells transfected with the TransIT-mRNA Transfection Kit were approximately 100% confluent at the time of harvest. |
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